07-585 Anti-dimethyl-Histone H3 (Arg2) Antibody

07-585
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H WB, PIA Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-585
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Arg2) Antibody
      Alternate Names
      • H3R2me2
      • Histone H3 (di methyl R2)
      References
      Product Information
      FormatSerum
      Presentationrabbit antiserum containing 0.05% sodium azide, before the addition of glycerol to 30%
      Applications
      ApplicationAnti-dimethyl-Histone H3 (Arg2) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H3 (Arg2) also known as H3R2me2, Histone H3 (di methyl R2) & has been validated in WB, PIA.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Biological Information
      Immunogenpeptide containing the sequence AdimRT, corresponding to dimethyl-arginine 2 of human histone H3
      HostRabbit
      Specificitydimethyl-arginine at residue 2 of histone H3
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      Species Reactivity NoteBroad species cross-reactivity expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      Modifications
      • Methylation
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular WeightMr 17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid-extracted proteins from chicken erythrocyte cells (Catalog # 13-107)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-dimethyl-Histone H3 (Arg2) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-dimethyl-Histone H3 (Arg2) Antibody Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Arg2) - 24419272441927
      Anti-dimethyl-Histone H3 (Arg2) - 22785132278513
      Anti-dimethyl-Histone H3 (Arg2) - 2695526955
      Anti-dimethyl-Histone H3 (Arg2) - DAM1531013DAM1531013
      Anti-dimethyl-Histone H3 (Arg2) - DAM1731499DAM1731499
      Anti-dimethyl-Histone H3 (Arg2) - DAM1776469DAM1776469
      Anti-dimethyl-Histone H3 (Arg2) -26944822694482
      Anti-dimethyl-Histone H3 (Arg2) -28511512851151
      Anti-dimethyl-Histone H3 (Arg2) -28603462860346
      Anti-dimethyl-Histone H3 (Arg2) Polyclonal Antibody2864357

      References

      Reference overviewApplicationSpeciesPub Med ID
      PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation.
      Kolodziej, S; Kuvardina, ON; Oellerich, T; Herglotz, J; Backert, I; Kohrs, N; Buscató, El; Wittmann, SK; Salinas-Riester, G; Bonig, H; Karas, M; Serve, H; Proschak, E; Lausen, J
      Nature communications  5  3995  2014

      Show Abstract
      Western Blotting24874575 24874575
      PELP1 oncogenic functions involve alternative splicing via PRMT6.
      Mann, M; Zou, Y; Chen, Y; Brann, D; Vadlamudi, R
      Molecular oncology  8  389-400  2014

      Show Abstract
      24447537 24447537
      The arginine methyltransferase PRMT6 regulates cell proliferation and senescence through transcriptional repression of tumor suppressor genes.
      Stein, C; Riedl, S; Rüthnick, D; Nötzold, RR; Bauer, UM
      Nucleic acids research  40  9522-33  2012

      Show Abstract
      22904088 22904088
      Systematic dissection of roles for chromatin regulators in a yeast stress response.
      Weiner, A; Chen, HV; Liu, CL; Rahat, A; Klien, A; Soares, L; Gudipati, M; Pfeffner, J; Regev, A; Buratowski, S; Pleiss, JA; Friedman, N; Rando, OJ
      PLoS biology  10  e1001369  2012

      Show Abstract
      22912562 22912562
      Cell cycle regulation by the PRMT6 arginine methyltransferase through repression of cyclin-dependent kinase inhibitors.
      Kleinschmidt, MA; de Graaf, P; van Teeffelen, HA; Timmers, HT
      PloS one  7  e41446  2012

      Show Abstract
      22916108 22916108
      Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans.
      Lin, H; Wang, Y; Wang, Y; Tian, F; Pu, P; Yu, Y; Mao, H; Yang, Y; Wang, P; Hu, L; Lin, Y; Liu, Y; Xu, Y; Chen, CD
      Cell research  20  899-907  2010

      Show Abstract
      20567262 20567262
      The solution structure of the first PHD finger of autoimmune regulator in complex with non-modified histone H3 tail reveals the antagonistic role of H3R2 methylation.
      Francesca Chignola, Massimiliano Gaetani, Ana Rebane, Tõnis Org, Luca Mollica, Chiara Zucchelli, Andrea Spitaleri, Valeria Mannella, Pärt Peterson, Giovanna Musco, Francesca Chignola, Massimiliano Gaetani, Ana Rebane, Tõnis Org, Luca Mollica, Chiara Zucchelli, Andrea Spitaleri, Valeria Mannella, Pärt Peterson, Giovanna Musco, Francesca Chignola, Massimiliano Gaetani, Ana Rebane, Tõnis Org, Luca Mollica, Chiara Zucchelli, Andrea Spitaleri, Valeria Mannella, Pärt Peterson, Giovanna Musco
      Nucleic acids research  37  2951-61  2009

      Show Abstract Full Text Article
      19293276 19293276
      PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation.
      Hyllus, D; Stein, C; Schnabel, K; Schiltz, E; Imhof, A; Dou, Y; Hsieh, J; Bauer, UM
      Genes & development  21  3369-80  2007

      Show Abstract Full Text Article
      Human18079182 18079182

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