04-077 Anti-dimethyl-Histone H3 (Arg17) Antibody, rabbit monoclonal

04-077
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt WB Rb Culture Supernatant Monoclonal Antibody
      Description
      Catalogue Number04-077
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Arg17) Antibody, rabbit monoclonal
      Alternate Names
      • H3R17me2
      • Histone H3 (di methyl R17)
      References
      Product Information
      FormatCulture Supernatant
      Presentation100 μL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide
      Applications
      ApplicationAnti-dimethyl-Histone H3 (Arg17) Antibody, rabbit is an antibody against dimethyl-Histone H3 (Arg17) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to human Histone H3 containing the sequence [AP-dimR-KQL] on which Arg17 is dimethylated.
      HostRabbit
      SpecificityRecognizes Histone H3 when dimethylated on Arg17
      IsotypeIgG
      Species Reactivity
      • Human
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • H3F3
      • MGC87782
      • H3.3B
      • MGC87783
      • H3F3B
      • H3.3A
      Modifications
      • Methylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight15kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-dimethyl-Histone H3 (Arg17) Antibody, rabbit monoclonal SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-dimethyl-Histone H3 (Arg17) Antibody, rabbit monoclonal Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Arg17), rabbit monoclonal3099984
      Anti-dimethyl-Histone H3 (Arg17), rabbit monoclonal - 20528762052876
      Anti-dimethyl-Histone H3 (Arg17), rabbit monoclonal - 22668122266812
      Anti-dimethyl-Histone H3 (Arg17), rabbit monoclonal - DAM1498202DAM1498202
      Anti-dimethyl-Histone H3 (Arg17), rabbit monoclonal -26211502621150

      References

      Reference overviewPub Med ID
      Methylation of Arginine Residues Interferes with Citrullination by Peptidylarginine Deiminases in vitro
      Raijmakers R, Zendman AJ, Egberts WV, Vossenaar ER, Raats J, Soede-Huijbregts C, Rutjes FP, van Veelen PA, Drijfhout JW, Pruijn GJ.
      Raijmakers, Reinout, et al  2007

      17303166 17303166
      Connections between epigenetic gene silencing and human disease.
      Moss, Timothy J and Wallrath, Lori L
      Mutat. Res., 618: 163-74 (2007)  2007

      Show Abstract
      17306846 17306846
      Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes.
      Bui, Hong-Thuy, et al.
      Reproduction, 133: 371-82 (2007)  2007

      Show Abstract
      17307905 17307905
      Methylation of histone H3 by coactivator-associated arginine methyltransferase 1
      Schurter, B T, et al
      Biochemistry, 40:5747-56 (2001)  2001

      11341840 11341840

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies