A part of MilliporeSigma

MABE637 | Anti-dimethyl Histone H3 (Lys4), clone RM135 Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, Mplex Rb Purified Monoclonal Antibody
      Catalogue NumberMABE637
      DescriptionAnti-dimethyl Histone H3 (Lys4), clone RM135 Antibody
      Alternate Names
      • Histone H3.3
      • Histone H3
      Background InformationHistone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
      Product Information
      PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
      ApplicationThis Anti-dimethyl Histone H3 (Lys4), clone RM135 Antibody is validated for use in Western Blotting, Multiplexing for the detection of dimethyl Histone H3.
      Key Applications
      • Western Blotting
      • Multiplexing
      Application NotesMultiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
      Biological Information
      ImmunogenA linear peptide corresponding to human Histone H3 dimethylated at Lys4.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis antibody reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylation in Histone H3.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • H3F3A
      • H3.3A
      • H3F3
      • PP781
      • H3F3B
      • H3.3B
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Recombinant HeLa protein and HeLa acid extract.

      Western Blotting Analysis (WB): 0.025 μg/mL of this antibody detected dimethyl Histone H3 (Lys4) in HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information