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07-369 | Anti-dimethyl-Histone H3 (Lys36) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt ChIP, WB Rb Serum Polyclonal Antibody
      Catalogue Number07-369
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Lys36) Antibody
      Alternate Names
      • H3K36me2
      • Histone H3 (di methyl K36)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • HeLa cell extract
      PresentationRabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide.
      Frozen at -20°C.
      ApplicationAnti-dimethyl-Histone H3 (Lys36) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H3 (Lys36) also known as H3K36me2, Histone H3 (di methyl K36) & has been validated in ChIP & WB.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesChromatin Immunoprecipitation:
      A previous lot was reported by an independent laboratory to immunoprecipitate formalin cross-linked chromatin containing Histone H3 methylated at lysine residue 36.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 31-41 (STGGV[dimethyl-K]KPHRY-C) of human, mouse, rat, and yeast histone H3 dimethylated on lysine 36, with a C-terminal cysteine added for conjugation purposes.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes dimethyl-Histone H3 (Lys36), Mr 17 kDa.
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteBroad, based on sequence conservation.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      • Methylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.


      TISSUE SPECIFICTY Expressed in testicular cells.

      DEVELOPMENTAL STAGE Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      Post-translational modification Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.

      Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.

      Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.

      Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.

      Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.

      Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

      Ubiquitinated By similarity.

      SEQUENCE SIMILARITIES Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on acid extracts from HeLa cells.

      Western Blot Analysis:
      1:2,000-1:10,000 dilution of this lot detected dimethyl-Histone H3 (Lys36) in acid extracts from HeLa cells (Catalog # 13-112) but not unmethylated recombinant Histone H3 (Catalog # 14-411).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Lys36) - 1966941 1966941
      Anti-dimethyl-Histone H3 (Lys36) - 22435 22435
      Anti-dimethyl-Histone H3 (Lys36) - 30257 30257
      Anti-dimethyl-Histone H3 (Lys36) - DAM1641102 DAM1641102
      Anti-dimethyl-Histone H3 (Lys36) - DAM1745326 DAM1745326
      Anti-dimethyl-Histone H3 (Lys36) - DAM1787879 DAM1787879
      Anti-dimethyl-Histone H3 (Lys36) - JBC1361840 JBC1361840
      Anti-dimethyl-Histone H3 (Lys36) - JBC1374254 JBC1374254
      Anti-dimethyl-Histone H3 (Lys36) - JBC1374254 JBC1374254
      Anti-dimethyl-Histone H3 (Lys36) - JBC1873475 JBC1873475

      References | 46 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      The Nucleosome Acidic Patch Regulates the H2B K123 Monoubiquitylation Cascade and Transcription Elongation in Saccharomyces cerevisiae.
      Cucinotta, CE; Young, AN; Klucevsek, KM; Arndt, KM
      PLoS genetics 11 e1005420 2015

      Show Abstract
      26241481 26241481
      Histone H3.3 and its proteolytically processed form drive a cellular senescence programme.
      Duarte, LF; Young, AR; Wang, Z; Wu, HA; Panda, T; Kou, Y; Kapoor, A; Hasson, D; Mills, NR; Ma'ayan, A; Narita, M; Bernstein, E
      Nature communications 5 5210 2014

      Show Abstract
      25394905 25394905
      dRYBP counteracts chromatin-dependent activation and repression of transcription.
      Fereres, S; Simón, R; Mohd-Sarip, A; Verrijzer, CP; Busturia, A
      PloS one 9 e113255 2014

      Show Abstract
      Western Blotting25415640 25415640
      The ASH1-RELATED3 SET-domain protein controls cell division competence of the meristem and the quiescent center of the Arabidopsis primary root.
      Kumpf, R; Thorstensen, T; Rahman, MA; Heyman, J; Nenseth, HZ; Lammens, T; Herrmann, U; Swarup, R; Veiseth, SV; Emberland, G; Bennett, MJ; De Veylder, L; Aalen, RB
      Plant physiology 166 632-43 2014

      Show Abstract
      25034019 25034019
      The demethylase JMJD2C localizes to H3K4me3-positive transcription start sites and is dispensable for embryonic development.
      Pedersen, MT; Agger, K; Laugesen, A; Johansen, JV; Cloos, PA; Christensen, J; Helin, K
      Molecular and cellular biology 34 1031-45 2014

      Show Abstract
      24396064 24396064
      Histone methyltransferase MMSET/NSD2 alters EZH2 binding and reprograms the myeloma epigenome through global and focal changes in H3K36 and H3K27 methylation.
      Popovic, R; Martinez-Garcia, E; Giannopoulou, EG; Zhang, Q; Zhang, Q; Ezponda, T; Shah, MY; Zheng, Y; Will, CM; Small, EC; Hua, Y; Bulic, M; Jiang, Y; Carrara, M; Calogero, RA; Kath, WL; Kelleher, NL; Wang, JP; Elemento, O; Licht, JD
      PLoS genetics 10 e1004566 2014

      Show Abstract
      Western Blotting25188243 25188243
      Distinct structural transitions of chromatin topological domains correlate with coordinated hormone-induced gene regulation.
      Le Dily, F; Baù, D; Pohl, A; Vicent, GP; Serra, F; Soronellas, D; Castellano, G; Wright, RH; Ballare, C; Filion, G; Marti-Renom, MA; Beato, M
      Genes & development 28 2151-62 2014

      Show Abstract
      25274727 25274727
      NuA4 links methylation of histone H3 lysines 4 and 36 to acetylation of histones H4 and H3.
      Ginsburg, DS; Anlembom, TE; Wang, J; Patel, SR; Li, B; Hinnebusch, AG
      The Journal of biological chemistry 289 32656-70 2014

      Show Abstract
      25301943 25301943
      Pharmacological inhibition of polycomb repressive complex-2 activity induces apoptosis in human colon cancer stem cells.
      Benoit, YD; Witherspoon, MS; Laursen, KB; Guezguez, A; Beauséjour, M; Beaulieu, JF; Lipkin, SM; Gudas, LJ
      Experimental cell research 319 1463-70 2013

      Show Abstract
      23588203 23588203
      Inhibition of PRC2 histone methyltransferase activity increases TRAIL-mediated apoptosis sensitivity in human colon cancer cells.
      Benoit, YD; Laursen, KB; Witherspoon, MS; Lipkin, SM; Gudas, LJ
      Journal of cellular physiology 228 764-72 2013

      Show Abstract
      Western Blotting23001792 23001792