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AB1584 | Anti-c-Fos Antibody

500 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R IHC, IH(P), WB Sh Purified Polyclonal Antibody
      Catalogue NumberAB1584
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-c-Fos Antibody
      Alternate Names
      • G0/G1 Switch Regulatory Protein 7
      Background Informationc-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.
      Product Information
      • Widely expressed
      PresentationPurified immunoglobulin. Lyophilized, no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
      ApplicationUse Anti-c-Fos Antibody (Sheep Polyclonal Antibody) validated in IHC, IHC(P), WB to detect c-Fos also known as G0/G1 Switch Regulatory Protein 7.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry: 1:500-1:4000, 2-8°C 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

      Recommended fixation is 4% PFA with 0.5% picric acid {Li et al 1999}; triton X-100 pretreatment in the block only; antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.

      Western blot: 10 μg/mL. Detects a band at approximately 55 kDa.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic c-fos peptide (residues Ser.Gly.Phe.Asn.Ala.Asp.Tyr.Glu.Ala. Ser.Ser.Ser.Arg.Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes c-fos.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
      Gene Symbol
      • FOS
      • G0S7
      • c-fos
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01100 # Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
      SIZE: 380 amino acids; 40695 Da
      SUBUNIT: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB (By similarity).
      SIMILARITY: SwissProt: P01100 ## Belongs to the bZIP family. Fos subfamily. & Contains 1 bZIP domain.
      Molecular Weight55 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to one year.

      Avoid repeated freeze/thaw cycles.

      During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      SHEEP ANTI-c-fos POLYCLONAL ANTIBODY - 2446719 2446719
      SHEEP ANTI-c-fos POLYCLONAL ANTIBODY - 2458945 2458945
      SHEEP ANTI-c-fos - 2147043 2147043
      SHEEP ANTI-c-fos -2610483 2610483
      SHEEP ANTI-c-fos -2624970 2624970
      SHEEP ANTI-c-fos -2685235 2685235
      SHEEP ANTI-c-fos -2712283 2712283
      SHEEP ANTI-c-fos -2718323 2718323
      SHEEP ANTI-c-fos -2726124 2726124
      SHEEP ANTI-c-fos -2736451 2736451
      SHEEP ANTI-c-fos -2739383 2739383
      SHEEP ANTI-c-fos -2807021 2807021
      SHEEP ANTI-c-fos POLYCLONAL ANTIBODY - 1984654 1984654
      SHEEP ANTI-c-fos POLYCLONAL ANTIBODY - 2091720 2091720
      SHEEP ANTI-c-fos POLYCLONAL ANTIBODY - 2287698 2287698
      SHEEP ANTI-c-fos POLYCLONAL ANTIBODY - 2326293 2326293
      SHEEP ANTI-c-fos POLYCLONAL ANTIBODY -2504796 2504796


      Reference overviewApplicationPub Med ID
      Inhibition of GSK3β improves hippocampus-dependent learning and rescues neurogenesis in a mouse model of fragile X syndrome.
      Guo, W; Murthy, AC; Zhang, L; Johnson, EB; Schaller, EG; Allan, AM; Zhao, X
      Human molecular genetics 21 681-91 2012

      Show Abstract
      Western Blotting22048960 22048960
      Changes in apoptosis-related protein (p53, Bax, Bcl-2 and Fos) expression with DNA fragmentation in the central nervous system in rats after closed head injury.
      J Lu, S Moochhala, C Kaur, E Ling
      Neuroscience letters 290 89-92 2000

      Show Abstract
      10936684 10936684
      Disinhibition of the rostral ventral medulla increases blood pressure and Fos expression in bulbospinal neurons.
      Minson, J B, et al.
      Brain Res., 646: 44-52 (1994) 1994

      Show Abstract
      8055340 8055340
      Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.
      van Straaten, F, et al.
      Proc. Natl. Acad. Sci. U.S.A., 80: 3183-7 (1983) 1983

      Show Abstract
      6574479 6574479

      Data Sheet


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      Life Science Research > Antibodies and Assays > Primary Antibodies