PC05L Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

PC05L
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC05L-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
          Catalogue NumberPC05L
          Brand Family Calbiochem®
          References
          ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
          Rouscher III, F.J., et al. 1988. Science 240, 1010.
          Sassone-Corsi, P., et al. 1988. Cell 54, 553.
          Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
          Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
          Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
          Verma, I. 1986. Trends Genet. 2, 93.
          Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
          Product Information
          FormLyophilized
          FormulationLyophilized from PBS, a volatile buffer, 100 µg BSA.
          Positive controlHep G2 or NIH 3T3 cells
          PreservativeNone
          Applications
          Application ReferencesGel Shift
          Guo, R-F., et al. 2002. Am. J. Pathol. 161, 275.
          Key Applications Immunofluorescence
          Immunoprecipitation
          Not Free-floating Sections
          Paraffin Sections
          Frozen Sections
          Gel Shift
          Immunoblotting (Western Blotting)
          Application NotesFrozen Sections (5-10 µg/ml)
          Immunoblotting (1-5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg per reaction)
          Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)
          Gel Shift (see comments and application references)
          Free-floating Sections (not recommended)
          Application CommentsFor gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-c-Fos (Ab-2) (4-17) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC05L

          References

          Reference overview
          DeTogni, P., et al. 1988. Cell Biol. 8, 2251.
          Rouscher III, F.J., et al. 1988. Science 240, 1010.
          Sassone-Corsi, P., et al. 1988. Cell 54, 553.
          Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
          Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
          Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
          Verma, I. 1986. Trends Genet. 2, 93.
          Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433

          Citations

          Title
        • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
        • Ren-Feng Guo, et al. (2002) Activator Protein-1 Actvation in Acute Lung Injury. American Journal of Pathology 161, 275-282.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-September-2015 JSW
          ApplicationFrozen Sections (5-10 µg/ml)
          Immunoblotting (1-5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg per reaction)
          Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)
          Gel Shift (see comments and application references)
          Free-floating Sections (not recommended)
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.
          BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlHep G2 or NIH 3T3 cells
          FormLyophilized
          FormulationLyophilized from PBS, a volatile buffer, 100 µg BSA.
          PreservativeNone
          CommentsFor gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.
          Toxicity Standard Handling
          ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
          Rouscher III, F.J., et al. 1988. Science 240, 1010.
          Sassone-Corsi, P., et al. 1988. Cell 54, 553.
          Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
          Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
          Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
          Verma, I. 1986. Trends Genet. 2, 93.
          Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
          Citation
        • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
        • Ren-Feng Guo, et al. (2002) Activator Protein-1 Actvation in Acute Lung Injury. American Journal of Pathology 161, 275-282.
        • Application referencesGel Shift
          Guo, R-F., et al. 2002. Am. J. Pathol. 161, 275.

          Related Products & Applications

          Related Products

          Catalog Number Description  
          PC05 Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Show Pricing & Availability

          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies