PC05 Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

PC05
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC05-100UG
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
          Catalogue NumberPC05
          Brand Family Calbiochem®
          References
          ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
          Rouscher III, F.J., et al. 1988. Science 240, 1010.
          Sassone-Corsi, P., et al. 1988. Cell 54, 553.
          Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
          Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
          Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
          Verma, I. 1986. Trends Genet. 2, 93.
          Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Positive controlHepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Not Free-floating Sections
          Paraffin Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesFrozen sections (5-10 µg/ml)
          Immunoblotting (1-5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/reaction)
          Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
          Free-floating Sections (not recommended)
          Application CommentsFor gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-c-Fos (Ab-2) (4-17) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC05

          References

          Reference overview
          DeTogni, P., et al. 1988. Cell Biol. 8, 2251.
          Rouscher III, F.J., et al. 1988. Science 240, 1010.
          Sassone-Corsi, P., et al. 1988. Cell 54, 553.
          Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
          Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
          Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
          Verma, I. 1986. Trends Genet. 2, 93.
          Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.

          Citations

          Title
        • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-September-2015 JSW
          ApplicationFrozen sections (5-10 µg/ml)
          Immunoblotting (1-5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/reaction)
          Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
          Free-floating Sections (not recommended)
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes v-fos and ~50-62 kDa c-fos proteins.
          BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation and development. Fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlHepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsFor gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Highly Toxic
          ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
          Rouscher III, F.J., et al. 1988. Science 240, 1010.
          Sassone-Corsi, P., et al. 1988. Cell 54, 553.
          Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
          Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
          Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
          Verma, I. 1986. Trends Genet. 2, 93.
          Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
          Citation
        • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
        • Related Products & Applications

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies