OP16L Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)

OP16L
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP16L-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          OP16L-500UG
          Retrieving availability...
          Limited AvailabilityLimited Availability
          Stocked 
          Discontinued
          Limited Quantities Available
          Available
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              Glass bottle 500 μg
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity needs to be mulitiple of
              Upon Order Completion More Information
              You Saved ()
               
              Request Pricing
              Description
              OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.
              Catalogue NumberOP16L
              Brand Family Calbiochem®
              SynonymsAnti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2
              References
              ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
              Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
              Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
              Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
              DiFiore, P.P., et al. 1987. Science 237, 178.
              Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
              Slamon, D.J., et al. Science 235, 177.
              Varley, J.M., et al. 1987. Oncogene 1, 423.
              Bargmann, C.I., et al. 1986. Nature 319, 226.
              Yamamoto, T., et al. 1986. Nature 319, 230.
              Blick, M., et al. 1984. Blood 64, 1234.
              Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
              Product Information
              FormLyophilized
              FormulationLyophilized from a volatile buffer, 100 µg BSA.
              Negative controlFisher rat embryo cells
              Positive controlB104-1-1 cells
              PreservativeNone
              Applications
              Application ReferencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Growth Inhibition Assays Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.
              Key Applications Immunocytochemistry
              Immunoprecipitation
              Frozen Sections
              Growth Inhibition Assay
              Immunoblotting (Western Blotting)
              Application NotesGrowth Inhibition Assays (see application references)
              Frozen Sections (5 µg/ml, see application references)
              Immunoblotting (see application references)
              Immunocytochemistry (5 µg/ml)
              Immunoprecipitation (1 µg/ml, see application references)
              Application CommentsStains frozen tissue sections of rat cells overexpressing the c-neu protein. This antibody does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
              Biological Information
              ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
              ImmunogenHuman
              Epitopewithin the extracellular domain
              Clone7.16.4
              HostMouse
              IsotypeIgG2a
              Species Reactivity
              • Human
              • Rat
              Antibody TypeMonoclonal Antibody
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) SDS

              Title

              Safety Data Sheet (SDS) 

              Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) Certificates of Analysis

              TitleLot Number
              OP16L

              References

              Reference overview
              Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
              Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
              Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
              Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
              DiFiore, P.P., et al. 1987. Science 237, 178.
              Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
              Slamon, D.J., et al. Science 235, 177.
              Varley, J.M., et al. 1987. Oncogene 1, 423.
              Bargmann, C.I., et al. 1986. Nature 319, 226.
              Yamamoto, T., et al. 1986. Nature 319, 230.
              Blick, M., et al. 1984. Blood 64, 1234.
              Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

              Citations

              Title
            • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
            • Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision16-November-2007 JSW
              SynonymsAnti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2
              ApplicationGrowth Inhibition Assays (see application references)
              Frozen Sections (5 µg/ml, see application references)
              Immunoblotting (see application references)
              Immunocytochemistry (5 µg/ml)
              Immunoprecipitation (1 µg/ml, see application references)
              DescriptionPurified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-Erb2/c-neu protein.
              BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
              HostMouse
              Immunogen speciesHuman
              ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
              Epitopewithin the extracellular domain
              Clone7.16.4
              IsotypeIgG2a
              Specieshuman, rat
              Positive controlB104-1-1 cells
              Negative controlFisher rat embryo cells
              FormLyophilized
              FormulationLyophilized from a volatile buffer, 100 µg BSA.
              PreservativeNone
              CommentsStains frozen tissue sections of rat cells overexpressing the c-neu protein. This antibody does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
              Storage +2°C to +8°C
              Do Not Freeze Ok to freeze
              Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
              Toxicity Standard Handling
              ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
              Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
              Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
              Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
              DiFiore, P.P., et al. 1987. Science 237, 178.
              Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
              Slamon, D.J., et al. Science 235, 177.
              Varley, J.M., et al. 1987. Oncogene 1, 423.
              Bargmann, C.I., et al. 1986. Nature 319, 226.
              Yamamoto, T., et al. 1986. Nature 319, 230.
              Blick, M., et al. 1984. Blood 64, 1234.
              Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
              Citation
            • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
            • Application referencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Growth Inhibition Assays Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.

              Related Products & Applications

              Related Products

              Catalog Number Description  
              OP16 Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) Show Pricing & Availability

              Categories

              Life Science Research > Antibodies and Assays > Primary Antibodies