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PC04 | Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb

PC04
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      PC04-100UG
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in Sk-BR-3 cells.
          Catalogue NumberPC04
          Brand Family Calbiochem®
          SynonymsAnti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus
          References
          ReferencesDi Fiore, P.P., et al. 1987. Science 237, 178.
          Slamon, D.J., et al. 1987. Science 235, 177.
          Varley, J.M., et al. 1987. Oncogene 1, 423.
          Bargmann, C.I., et al. 1986. Nature 319 226.
          Yamamoto, T., et al. 1986. Nature 319, 230.
          Blick, M., et al. 1984. Blood 64, 1234.
          Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
          Negative controlNormal human fibroblasts
          Positive controlSK-BR-3 cells
          PreservativeNone
          Applications
          Application ReferencesParaffin Sections King, B.L., et al. 1992. Am. J. Pathol 140, 23.
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Application NotesImmunoblotting (1-5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see application references)
          Application CommentsAntibody should be titrated for optimal results in individual sample types.
          Biological Information
          Immunogena synthetic peptide (LARLLDIDETEYHAD) corresponding to amino acids 866-880 from the kinase domain of human c-Neu
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          PC04

          References

          Reference overview
          Di Fiore, P.P., et al. 1987. Science 237, 178.
          Slamon, D.J., et al. 1987. Science 235, 177.
          Varley, J.M., et al. 1987. Oncogene 1, 423.
          Bargmann, C.I., et al. 1986. Nature 319 226.
          Yamamoto, T., et al. 1986. Nature 319, 230.
          Blick, M., et al. 1984. Blood 64, 1234.
          Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-October-2007 RFH
          SynonymsAnti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus
          ApplicationImmunoblotting (1-5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see application references)
          DescriptionPurified rabbit polyclonal antibody. Recognizes the ~185 kDa native and denatured forms of c-ErbB2/c-Neu.
          BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification may be a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. Neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (LARLLDIDETEYHAD) corresponding to amino acids 866-880 from the kinase domain of human c-Neu
          IsotypeIgG
          Specieshuman, rat
          Positive controlSK-BR-3 cells
          Negative controlNormal human fibroblasts
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsAntibody should be titrated for optimal results in individual sample types.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesDi Fiore, P.P., et al. 1987. Science 237, 178.
          Slamon, D.J., et al. 1987. Science 235, 177.
          Varley, J.M., et al. 1987. Oncogene 1, 423.
          Bargmann, C.I., et al. 1986. Nature 319 226.
          Yamamoto, T., et al. 1986. Nature 319, 230.
          Blick, M., et al. 1984. Blood 64, 1234.
          Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
          Application referencesParaffin Sections King, B.L., et al. 1992. Am. J. Pathol 140, 23.