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ABT170 | Anti-alpha Tubulin Antibody, nontyrosinated

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Porcine, H, R WB, ELISA, ICC Rb Serum Polyclonal Antibody
      Catalogue NumberABT170
      DescriptionAnti-alpha Tubulin Antibody, nontyrosinated
      Alternate Names
      • Tubulin alpha-1A chain
      • Alpha-tubulin 3
      • Tubulin B-alpha-1
      • Tubulin alpha-3 chain
      Background InformationMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
      Product Information
      PresentationRabbit polyclonal serum in buffer containing serum with 0.05% sodium azide.
      ApplicationDetect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin and not tyrosinated alpha Tubulin from purified porcine alpha Tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

      ELISA Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human nontyrosinated alpha Tubulin.
      SpecificityThis antibody detects the nontyrosinated form of alpha Tubulin.
      Species Reactivity
      • Mouse
      • Porcine
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse and Porcine. Predicted to react with Human and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBA1A
      • TUBA3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~51 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, nontyrosinated in 10 µg of PCA treated NIH/3T3 cell lysate and demonstrated a loss of signal in untreated NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-alpha Tubulin, nontyrosinated - Q2290313 Q2290313
      Anti-alpha Tubulin, nontyrosinated -2649272 2649272
      Anti-alpha Tubulin, nontyrosinated -2702144 2702144
      Anti-alpha Tubulin, nontyrosinated -2784650 2784650
      Anti-alpha Tubulin, nontyrosinated -2803662 2803662


      Reference overviewPub Med ID
      Distinct populations of microtubules: tyrosinated and nontyrosinated alpha tubulin are distributed differently in vivo.
      Gundersen, G G, et al.
      Cell, 38: 779-89 (1984) 1984

      Show Abstract
      6386177 6386177

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      Life Science Research > Antibodies and Assays > Primary Antibodies