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ABE28 | Anti-acetyl-MeCP2 (Lys464) Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R DB, WB, ICC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABE28
      DescriptionAnti-acetyl-MeCP2 (Lys464) Antibody
      Alternate Names
      • Methyl-CpG-binding protein 2
      • MeCp-2 protein
      • MeCp2
      Background InformationDNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
      Product Information
      FormatAffinity Purified
      • Non-acetylated and acetylated MeCP2 (Lys464) peptides.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationUse Anti-acetyl-MeCP2 (Lys464) Antibody (Rabbit Polyclonal Antibody) validated in DB, WB, ICC to detect acetyl-MeCP2 (Lys464) also known as Methyl-CpG-binding protein 2, MeCp-2 protein.
      Key Applications
      • Dot Blot
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Immunoprecipitation Analysis: A representative lot from an independent laboratory detected MeCP2 in 293 cell lysate derived from p300 co-transfected 293 cells. (Prof. Sassone-Corsi, Center for Epigenetics and Metabolism, University of California, Irvine.)

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected MeCP2 in mouse cortical neurons. (Prof. Sassone-Corsi, Center for Epigenetics and Metabolism, University of California, Irvine.)

      Alexa Fluor® is a registered trademark of Molecular Probes Inc.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse MeCP2 acetylated at Lys464.
      EpitopeAcetylated lysine 464
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes MeCP2 acetylated at Lysine 464.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryDNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
      Gene Symbol
      • Mecp2
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.

      SUBUNIT STRUCTURE: Interacts with CDKL5. Interacts with FNBP3.

      SUBCELLULAR LOCATION: Nucleus. Note: Colocalized with methyl-CpG in the genome.
      Molecular Weight~75 kDa observed. Two isoforms (A and B) with calculated molecular weights of ~52 kDa and 53 kDa are known to exist. This antibody should detect either isoform.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot using non-acetylated and acetylated MeCP2 (Lys464) peptides.

      Dot Blot Analysis: 1 µg/mL of this antibody detected MeCP2 using non-acetylated and acetylated MeCP2 (Lys464) peptides.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-MeCP2 (Lys464) - 2289164 2289164
      Anti-acetyl-MeCP2 (Lys464) - 2361552 2361552
      Anti-acetyl-MeCP2 (Lys464) - NRG1950714 NRG1950714
      Anti-acetyl-MeCP2 (Lys464) -2806001 2806001


      Reference overviewPub Med ID
      SIRT1-mediated deacetylation of MeCP2 contributes to BDNF expression.
      Zocchi, L; Sassone-Corsi, P
      Epigenetics 7 695-700 2012

      Show Abstract
      22677942 22677942


      New Products: Volume 3, 2012
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.