06-933 Anti-acetyl-Lysine Antibody

06-933
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt WB, IP Rb Serum Polyclonal Antibody
      Description
      Catalogue Number06-933
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Lysine Antibody
      Background InformationIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
      References
      Product Information
      FormatSerum
      Control
      • TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
      PresentationRabbit IgG serum containing 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-acetyl-Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of acetyl-Lysine & has been validated in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: 5 μL of a previous lot immunoprecipitated in vitro acetylated recombinant p300.

      Western Blot Analysis: A 1:1000 dilution of the previous lot of the antibody detected:
      • Acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5 μM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B).
      • GST-p53 (100 ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C).
      Biological Information
      ImmunogenA mixture of chemically acetylated antigens.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAcetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA.
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteBroad species reactivity expected.
      Antibody TypePolyclonal Antibody
      Modifications
      • Acetylation
      Purification MethodAntiserum
      Molecular WeightDependent upon the molecular weight of the lysine acetylated protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on acid-extracted proteins from sodium butyrate treated HeLa cells, chemically acetylated BSA, acetylated histones and to a lesser degree, other proteins in trichostatin A treated Cos-1 cell lysate.

      Western Blot Analysis: 1:1000-1:2000 dilution of this antibody detected:
      • Acetylated histones in acid-extracted proteins from sodium butyrate (5 mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A).
      • Chemically acetylated BSA (10 ng) in a dot blot assay.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Lysine - 24651952465195
      Anti-acetyl-Lysine - 1874118741
      Anti-acetyl-Lysine - 1921219212
      Anti-acetyl-Lysine - 1970219702
      Anti-acetyl-Lysine - 2187321873
      Anti-acetyl-Lysine - 2640926409
      Anti-acetyl-Lysine - 31886233188623
      Anti-acetyl-Lysine -27436372743637
      Anti-acetyl-Lysine -27456092745609
      Anti-acetyl-Lysine -27571392757139

      References

      Reference overviewApplicationSpeciesPub Med ID
      Post transcriptional control of the epigenetic stem cell regulator PLZF by sirtuin and HDAC deacetylases.
      McConnell, MJ; Durand, L; Langley, E; Coste-Sarguet, L; Zelent, A; Chomienne, C; Kouzarides, T; Licht, JD; Guidez, F
      Epigenetics & chromatin  8  38  2015

      Show Abstract
      26405459 26405459
      Camello, a novel family of Histone Acetyltransferases that acetylate histone H4 and is essential for zebrafish development.
      Karmodiya, K; Anamika, K; Muley, V; Pradhan, SJ; Bhide, Y; Galande, S
      Scientific reports  4  6076  2014

      Show Abstract
      Western Blotting25123547 25123547
      Transcriptional repressor NIR interacts with the p53-inhibiting ubiquitin ligase MDM2.
      Heyne, K; Förster, J; Schüle, R; Roemer, K
      Nucleic acids research  42  3565-79  2014

      Show Abstract
      24413661 24413661
      Myofibrillar Ca(2+) sensitivity is uncoupled from troponin I phosphorylation in hypertrophic obstructive cardiomyopathy due to abnormal troponin T.
      Bayliss, CR; Jacques, AM; Leung, MC; Ward, DG; Redwood, CS; Gallon, CE; Copeland, O; McKenna, WJ; Dos Remedios, C; Marston, SB; Messer, AE
      Cardiovascular research  97  500-8  2013

      Show Abstract
      23097574 23097574
      mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
      Mitchell, L; Huard, S; Cotrut, M; Pourhanifeh-Lemeri, R; Steunou, AL; Hamza, A; Lambert, JP; Zhou, H; Ning, Z; Basu, A; Côté, J; Figeys, DA; Baetz, K
      Proceedings of the National Academy of Sciences of the United States of America  110  E1641-50  2013

      Show Abstract
      23572591 23572591
      Gli2 acetylation at lysine 757 regulates hedgehog-dependent transcriptional output by preventing its promoter occupancy.
      Coni, S; Antonucci, L; D'Amico, D; Di Magno, L; Infante, P; De Smaele, E; Giannini, G; Di Marcotullio, L; Screpanti, I; Gulino, A; Canettieri, G
      PloS one  8  e65718  2013

      Show Abstract
      23762415 23762415
      Tasquinimod Is an Allosteric Modulator of HDAC4 survival signaling within the compromised cancer microenvironment.
      Isaacs, JT; Antony, L; Dalrymple, SL; Brennen, WN; Gerber, S; Hammers, H; Wissing, M; Kachhap, S; Luo, J; Xing, L; Björk, P; Olsson, A; Björk, A; Leanderson, T
      Cancer research  73  1386-99  2013

      Show Abstract
      23149916 23149916
      Modulation of histone deacetylase 6 (HDAC6) nuclear import and tubulin deacetylase activity through acetylation.
      Liu, Y; Peng, L; Seto, E; Huang, S; Qiu, Y
      The Journal of biological chemistry  287  29168-74  2012

      Show Abstract
      Western Blotting22778253 22778253
      Identifying acetylated proteins in mitosis.
      Carol Chuang,Li-Yuan Yu-Lee
      Methods in molecular biology (Clifton, N.J.)  909  2012

      Show Abstract
      22903717 22903717
      Targeted acetylation of NF-kappaB/RelA and histones by epigenetic drugs reduces post-ischemic brain injury in mice with an extended therapeutic window.
      Lanzillotta, Annamaria, et al.
      Neurobiol. Dis., 49C: 177-189 (2012)  2012

      Show Abstract
      22971966 22971966