07-328 Anti-acetyl-Histone H4 (Lys8) Antibody

07-328
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Yeast (S. cerevisiae) IP, WB, DB, ChIP-seq Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-328
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H4 (Lys8) Antibody
      Alternate Names
      • H4K8Ac
      • Histone H4 (acetyl K8)
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatSerum
      Control
      • Sodium butyrate treated HeLa cells
      PresentationUnpurified rabbit polyclonal in serum with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-acetyl-Histone H4 (Lys8) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, IP, WB, DB, ChIP-seq to detect acetyl-Histone H4 (Lys8) also known as H4K8Ac, Histone H4 (acetyl K8).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Dot Blot
      • ChIP-seq
      Biological Information
      ImmunogenPeptide corresponding to amino acids 3-13 of yeast Histone H4 (RGKGGAcKGLGKG-C) in which lysine 8 is acetylated.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes acetyl-Histone H4 (Lys8).
      IsotypeIgG
      Species Reactivity
      • Human
      • Yeast (S. cerevisiae)
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl Histone H4 (Lys8) - 24334452433445
      Anti-acetyl Histone H4 (Lys8) - 20197402019740
      Anti-acetyl Histone H4 (Lys8) - 20759562075956
      Anti-acetyl Histone H4 (Lys8) - 21899522189952
      Anti-acetyl Histone H4 (Lys8) - 23016512301651
      Anti-acetyl Histone H4 (Lys8) - 24996982499698
      Anti-acetyl Histone H4 (Lys8) - DAM1676174DAM1676174
      Anti-acetyl Histone H4 (Lys8) - DAM1731490DAM1731490
      Anti-acetyl Histone H4 (Lys8) -25576412557641
      Anti-acetyl Histone H4 (Lys8) -25824762582476

      References

      Reference overviewApplicationSpeciesPub Med ID
      Ethanol exposure induces neonatal neurodegeneration by enhancing CB1R Exon1 histone H4K8 acetylation and up-regulating CB1R function causing neurobehavioral abnormalities in adult mice.
      Subbanna, S; Nagre, NN; Umapathy, NS; Pace, BS; Basavarajappa, BS
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Show Abstract
      25609594 25609594
      Characterization of BRD4 during mammalian postmeiotic sperm development.
      Bryant, JM; Donahue, G; Wang, X; Meyer-Ficca, M; Luense, LJ; Weller, AH; Bartolomei, MS; Blobel, GA; Meyer, RG; Garcia, BA; Berger, SL
      Molecular and cellular biology  35  1433-48  2015

      Show Abstract
      Immunofluorescence25691659 25691659
      Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline.
      Sin, HS; Kartashov, AV; Hasegawa, K; Barski, A; Namekawa, SH
      BMC biology  13  53  2015

      Show Abstract
      26198001 26198001
      Long noncoding RNA PANDA and scaffold-attachment-factor SAFA control senescence entry and exit.
      Puvvula, PK; Desetty, RD; Pineau, P; Marchio, A; Moon, A; Dejean, A; Bischof, O
      Nature communications  5  5323  2014

      Show Abstract
      25406515 25406515
      Lunasin sensitivity in non-small cell lung cancer cells is linked to suppression of integrin signaling and changes in histone acetylation.
      Inaba, J; McConnell, EJ; Davis, KR
      International journal of molecular sciences  15  23705-24  2014

      Show Abstract
      Western Blotting25530619 25530619
      Rational design and validation of a Tip60 histone acetyltransferase inhibitor.
      Gao, C; Bourke, E; Scobie, M; Famme, MA; Koolmeister, T; Helleday, T; Eriksson, LA; Lowndes, NF; Brown, JA
      Scientific reports  4  5372  2014

      Show Abstract
      Western BlottingChicken24947938 24947938
      Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice.
      Subbanna, S; Nagre, NN; Shivakumar, M; Umapathy, NS; Psychoyos, D; Basavarajappa, BS
      Neuroscience  258  422-32  2014

      Show Abstract
      Immunoprecipitation24300108 24300108
      Histone methylation has dynamics distinct from those of histone acetylation in cell cycle reentry from quiescence.
      Mews, P; Zee, BM; Liu, S; Donahue, G; Garcia, BA; Berger, SL
      Molecular and cellular biology  34  3968-80  2014

      Show Abstract
      25154414 25154414
      Crosstalk between NSL histone acetyltransferase and MLL/SET complexes: NSL complex functions in promoting histone H3K4 di-methylation activity by MLL/SET complexes.
      Zhao, X; Su, J; Wang, F; Liu, D; Ding, J; Yang, Y; Conaway, JW; Conaway, RC; Cao, L; Wu, D; Wu, M; Cai, Y; Jin, J
      PLoS genetics  9  e1003940  2013

      Show Abstract
      24244196 24244196
      Brd4 is displaced from HPV replication factories as they expand and amplify viral DNA.
      Sakakibara, N; Chen, D; Jang, MK; Kang, DW; Luecke, HF; Wu, SY; Chiang, CM; McBride, AA
      PLoS pathogens  9  e1003777  2013

      Show Abstract
      24278023 24278023

      Posters

      Title
      Histone Modifications