07-327 Anti-acetyl-Histone H4 (Lys5) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, DB, ICC, ChIP-seq, ChIP Rb Serum Polyclonal Antibody
      Catalogue Number07-327
      DescriptionAnti-acetyl-Histone H4 (Lys5) Antibody
      Alternate Names
      • Histone H4
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      Product Information
      • HeLa acid extract
      PresentationImmunodepleted rabbit serum with 0.05% soduim azide and 30% glycerol.
      ApplicationUse Anti-acetyl-Histone H4 (Lys5) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, ICC, ChIP-seq, ChIP to detect acetyl-Histone H4 (Lys5) also known as H4K5Ac, Histone H4 (acetyl K5).
      Key Applications
      • Western Blotting
      • Dot Blot
      • Immunocytochemistry
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected acetyl-Histone H4 in HeLa and A431 cells.

      Dot Blot Specificity Analysis: A representative lot detected acetyl-histone H4 (Lys5) peptides, but not unacetylated-histone H4 (Lys4) peptides or other peptides corresponding to modified histones.

      Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated acetyl-Histone H4 in HeLa S3 chromatin.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of Histone H4 acetylated at Lys5.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the N-terminus of Histone H4 acetylated at Lys5.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human based on 100% sequence homology. This antibody is expected to demonstrate broad species cross-reactivity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
      Gene Symbol
      • HIST1H4A
      • H4/A
      • H4FA
      • HIST1H4B
      • H4/I
      • H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • H4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      • Acetylation
      Purification MethodPurified by immunodepletion
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus. Chromosome.
      Molecular Weight~13 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H4 in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H4 (Lys5) - 24468522446852
      Anti-acetyl-Histone H4 (Lys5) - 19112631911263
      Anti-acetyl-Histone H4 (Lys5) - 19924401992440
      Anti-acetyl-Histone H4 (Lys5) - 20197412019741
      Anti-acetyl-Histone H4 (Lys5) - 22025452202545
      Anti-acetyl-Histone H4 (Lys5) - 2229522295
      Anti-acetyl-Histone H4 (Lys5) - 23665612366561
      Anti-acetyl-Histone H4 (Lys5) - 3041730417
      Anti-acetyl-Histone H4 (Lys5) - DAM1503383DAM1503383
      Anti-acetyl-Histone H4 (Lys5) - DAM1514009DAM1514009


      Reference overviewApplicationPub Med ID
      Characterization of BRD4 during mammalian postmeiotic sperm development.
      Bryant, JM; Donahue, G; Wang, X; Meyer-Ficca, M; Luense, LJ; Weller, AH; Bartolomei, MS; Blobel, GA; Meyer, RG; Garcia, BA; Berger, SL
      Molecular and cellular biology  35  1433-48  2015

      Show Abstract
      Immunofluorescence25691659 25691659
      AtEAF1 is a potential platform protein for Arabidopsis NuA4 acetyltransferase complex.
      Bieluszewski, T; Galganski, L; Sura, W; Bieluszewska, A; Abram, M; Ludwikow, A; Ziolkowski, PA; Sadowski, J
      BMC plant biology  15  75  2015

      Show Abstract
      25849764 25849764
      Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs.
      Laham-Karam, N; Lalli, M; Leinonen, N; Ylä-Herttuala, S
      Molecular therapy. Nucleic acids  4  e243  2015

      Show Abstract
      25988242 25988242
      Altered nucleosome positioning at the transcription start site and deficient transcriptional initiation in Friedreich ataxia.
      Chutake, YK; Costello, WN; Lam, C; Bidichandani, SI
      The Journal of biological chemistry  289  15194-202  2014

      Show Abstract
      24737321 24737321
      Comparative analysis of genome-wide chromosomal histone modification patterns in maize cultivars and their wild relatives.
      He, S; Yan, S; Wang, P; Zhu, W; Wang, X; Shen, Y; Shao, K; Xin, H; Li, S; Li, L
      PloS one  9  e97364  2014

      Show Abstract
      Immunocytochemistry24819606 24819606
      Histone methylation has dynamics distinct from those of histone acetylation in cell cycle reentry from quiescence.
      Mews, P; Zee, BM; Liu, S; Donahue, G; Garcia, BA; Berger, SL
      Molecular and cellular biology  34  3968-80  2014

      Show Abstract
      25154414 25154414
      Establishment of regions of genomic activity during the Drosophila maternal to zygotic transition.
      Li, XY; Harrison, MM; Villalta, JE; Kaplan, T; Eisen, MB
      eLife  3  2014

      Show Abstract
      25313869 25313869
      Epstein-Barr virus-mediated transformation of B cells induces global chromatin changes independent to the acquisition of proliferation.
      Hernando, H; Islam, AB; Rodríguez-Ubreva, J; Forné, I; Ciudad, L; Imhof, A; Shannon-Lowe, C; Ballestar, E
      Nucleic acids research  42  249-63  2014

      Show Abstract
      Western Blotting24097438 24097438
      Crosstalk between NSL histone acetyltransferase and MLL/SET complexes: NSL complex functions in promoting histone H3K4 di-methylation activity by MLL/SET complexes.
      Zhao, X; Su, J; Wang, F; Liu, D; Ding, J; Yang, Y; Conaway, JW; Conaway, RC; Cao, L; Wu, D; Wu, M; Cai, Y; Jin, J
      PLoS genetics  9  e1003940  2013

      Show Abstract
      24244196 24244196
      FOSL1 controls the assembly of endothelial cells into capillary tubes by direct repression of αv and β3 integrin transcription.
      Evellin, S; Galvagni, F; Zippo, A; Neri, F; Orlandini, M; Incarnato, D; Dettori, D; Neubauer, S; Kessler, H; Wagner, EF; Oliviero, S
      Molecular and cellular biology  33  1198-209  2013

      Show Abstract
      Western Blotting, Immunofluorescence23319049 23319049


      Histone Modifications