07-329 Anti-acetyl-Histone H4 (Lys16) Antibody

07-329
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, Mplex, PIA, DB, ChIP-seq, ChIP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-329
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H4 (Lys16) Antibody
      Alternate Names
      • Histone H4 Acetylated on Lysine 16
      • H4K16Ac
      • Histone H4 (acetyl K16)
      • H4 histone family
      • member A
      • H4a
      Background InformationHistone H4, Acetylated on Lysine 16 (UniProt: P62805; also known as H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a) is encoded by the HIST1H4A (also known as H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, HIST1H4I, H4/N, HIST1H4B) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 features a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins. Chromosomal aberrations involving histone H4 is a cause of B-cell non-Hodgkin lymphomas.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-acetyl-Histone H4 (Lys16) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16 & has been published and validated in ChIP, WB, Mplex, PIA, DB, ChIP-seq.
      Key Applications
      • Western Blotting
      • Multiplexing
      • Peptide Inhibition Assay
      • Dot Blot
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis: 1 ug from a representative lot immunoprecipitated Acetyl-Histone H4 (Lys16) in HeLa chromatin.

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in HeLa, A431, HUVEC, and NIH/3T3 cell lines.

      Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in

      an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H4 acetylated on Lysine 16.
      EpitopeN-terminus (Lys 16)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human Histone H4 acetylated on Lysine 16.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity Note Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • HIST1H4I
      • H4/N
      • HIST1H4B
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from Sodium Butyrate treated HeLa cells.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Acetyl-Histone H4 (Lys16) in lysates from Sodium Butyrate treated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8° C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Acetyl-Histone H4 (Lys16)3068450
      Anti-Acetyl-Histone H4 (Lys16) - 31707933170793
      Anti-Acetyl-Histone H4 (Lys16) -25746022574602
      Anti-Acetyl-Histone H4 (Lys16) -26399262639926
      Anti-Acetyl-Histone H4 (Lys16) -26780562678056
      Anti-Acetyl-Histone H4 (Lys16) -27167532716753
      Anti-Acetyl-Histone H4 (Lys16) -27804982780498
      Anti-Acetyl-Histone H4 (Lys16) Polyclonal Antibody3013289
      Anti-Acetyl-Histone H4 (Lys16) Polyclonal Antibody2960455
      Anti-Acetyl-Histone H4 (Lys16) Polyclonal Antibody2919704

      References

      Reference overviewApplicationSpeciesPub Med ID
      TP53 engagement with the genome occurs in distinct local chromatin environments via pioneer factor activity.
      Sammons, MA; Zhu, J; Drake, AM; Berger, SL
      Genome research  25  179-88  2015

      Show Abstract
      25391375 25391375
      Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.
      Lim, KK; Ong, TY; Tan, YR; Yang, EG; Ren, B; Seah, KS; Yang, Z; Tan, TS; Dymock, BW; Chen, ES
      Scientific reports  5  14064  2015

      Show Abstract
      26369364 26369364
      Ancestral Chromatin Configuration Constrains Chromatin Evolution on Differentiating Sex Chromosomes in Drosophila.
      Zhou, Q; Bachtrog, D
      PLoS genetics  11  e1005331  2015

      Show Abstract
      26114585 26114585
      The specification and global reprogramming of histone epigenetic marks during gamete formation and early embryo development in C. elegans.
      Samson, M; Jow, MM; Wong, CC; Fitzpatrick, C; Aslanian, A; Saucedo, I; Estrada, R; Ito, T; Park, SK; Yates, JR; Chu, DS
      PLoS genetics  e1004588  2014

      Show Abstract
      Immunofluorescence25299455 25299455
      Uncoupling transcription from covalent histone modification.
      Zhang, H; Gao, L; Anandhakumar, J; Gross, DS
      PLoS genetics  10  e1004202  2014

      Show Abstract
      24722509 24722509
      Lunasin sensitivity in non-small cell lung cancer cells is linked to suppression of integrin signaling and changes in histone acetylation.
      Inaba, J; McConnell, EJ; Davis, KR
      International journal of molecular sciences  15  23705-24  2014

      Show Abstract
      Western Blotting25530619 25530619
      The chromatin landscape of Drosophila: comparisons between species, sexes, and chromosomes.
      Brown, EJ; Bachtrog, D
      Genome research  24  1125-37  2014

      Show Abstract
      24840603 24840603
      MOF-associated complexes ensure stem cell identity and Xist repression.
      Chelmicki, T; Dündar, F; Turley, MJ; Khanam, T; Aktas, T; Ramírez, F; Gendrel, AV; Wright, PR; Videm, P; Backofen, R; Heard, E; Manke, T; Akhtar, A
      eLife  3  e02024  2014

      Show Abstract
      24842875 24842875
      Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory.
      Hait, NC; Wise, LE; Allegood, JC; O'Brien, M; Avni, D; Reeves, TM; Knapp, PE; Lu, J; Luo, C; Miles, MF; Milstien, S; Lichtman, AH; Spiegel, S
      Nature neuroscience  17  971-80  2014

      Show Abstract
      Western BlottingMouse24859201 24859201
      LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16.
      Yin, F; Lan, R; Zhang, X; Zhu, L; Chen, F; Xu, Z; Liu, Y; Ye, T; Sun, H; Lu, F; Zhang, H
      Molecular and cellular biology  34  158-79  2014

      Show Abstract
      24190971 24190971

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

      Posters

      Title
      Histone Modifications

      FAQ

      QuestionAnswer
      What is the concentration of this antibody?We are not able to quantitate antiserum.