07-595 Anti-acetyl-Histone H4 (Lys12) Antibody

07-595
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Yeast (S. cerevisiae) DB, WB, ChIP-seq Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-595
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H4 (Lys12) Antibody
      Alternate Names
      • H4K12Ac
      • Histone H4 (acetyl K12)
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatSerum
      Control
      • Acid extracted proteins from HeLa cells treated with sodium butyrate
      PresentationDepleted rabbit antiserum in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationUse Anti-acetyl-Histone H4 (Lys12) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, DB, WB, ChIP-seq to detect acetyl-Histone H4 (Lys12) also known as H4K12Ac, Histone H4 (acetyl K12).
      Key Applications
      • Dot Blot
      • Western Blotting
      • ChIP-seq
      Biological Information
      Immunogenpeptide containing the sequence (LGAcKGG) corresponding to lysine 12 acetylation of yeast Histone H4.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone H4 acetylated on lysine 12.
      IsotypeIgG
      Species Reactivity
      • Human
      • Yeast (S. cerevisiae)
      Species Reactivity NoteBroad species cross-reactivity expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST2H4B
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • H4/o
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/C
      • H4/D
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodImmunodepleted Serum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracted proteins from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H4 (Lys12) - 24441362444136
      Anti-acetyl-Histone H4 (Lys12) - 24628562462856
      Anti-acetyl-Histone H4 (Lys12) - 19848451984845
      Anti-acetyl-Histone H4 (Lys12) - 20197232019723
      Anti-acetyl-Histone H4 (Lys12) - 23291232329123
      Anti-acetyl-Histone H4 (Lys12) - 2698326983
      Anti-acetyl-Histone H4 (Lys12) - 2888528885
      Anti-acetyl-Histone H4 (Lys12) - 32091643209164
      Anti-acetyl-Histone H4 (Lys12) - DAM1655221DAM1655221
      Anti-acetyl-Histone H4 (Lys12) - JBC1865968JBC1865968

      References

      Reference overviewApplicationSpeciesPub Med ID
      Methylation of histone H3 lysine 9 occurs during translation.
      Rivera, C; Saavedra, F; Alvarez, F; Díaz-Celis, C; Ugalde, V; Li, J; Forné, I; Gurard-Levin, ZA; Almouzni, G; Imhof, A; Loyola, A
      Nucleic acids research  43  9097-106  2015

      Show Abstract
      26405197 26405197
      Characterization of BRD4 during mammalian postmeiotic sperm development.
      Bryant, JM; Donahue, G; Wang, X; Meyer-Ficca, M; Luense, LJ; Weller, AH; Bartolomei, MS; Blobel, GA; Meyer, RG; Garcia, BA; Berger, SL
      Molecular and cellular biology  35  1433-48  2015

      Show Abstract
      Immunofluorescence25691659 25691659
      H4K12ac is regulated by estrogen receptor-alpha and is associated with BRD4 function and inducible transcription.
      Nagarajan, S; Benito, E; Fischer, A; Johnsen, SA
      Oncotarget  6  7305-17  2015

      Show Abstract
      25788266 25788266
      TELP, a sensitive and versatile library construction method for next-generation sequencing.
      Peng, X; Wu, J; Brunmeir, R; Kim, SY; Zhang, Q; Ding, C; Han, W; Xie, W; Xu, F
      Nucleic acids research  43  e35  2015

      Show Abstract
      Mouse25223787 25223787
      The complex pattern of epigenomic variation between natural yeast strains at single-nucleosome resolution.
      Filleton, F; Chuffart, F; Nagarajan, M; Bottin-Duplus, H; Yvert, G
      Epigenetics & chromatin  8  26  2015

      Show Abstract
      26229551 26229551
      Histone H3 K27 acetylation marks a potent enhancer element on the adipogenic master regulator gene Pparg2.
      Ramlee, MK; Zhang, Q; Idris, M; Peng, X; Sim, CK; Han, W; Xu, F
      Cell cycle (Georgetown, Tex.)  13  3414-22  2014

      Show Abstract
      25485585 25485585
      Uncoupling transcription from covalent histone modification.
      Zhang, H; Gao, L; Anandhakumar, J; Gross, DS
      PLoS genetics  10  e1004202  2014

      Show Abstract
      24722509 24722509
      Lunasin sensitivity in non-small cell lung cancer cells is linked to suppression of integrin signaling and changes in histone acetylation.
      Inaba, J; McConnell, EJ; Davis, KR
      International journal of molecular sciences  15  23705-24  2014

      Show Abstract
      Western Blotting25530619 25530619
      Deletion of a conserved cis-element in the Ifng locus highlights the role of acute histone acetylation in modulating inducible gene transcription.
      Balasubramani, A; Winstead, CJ; Turner, H; Janowski, KM; Harbour, SN; Shibata, Y; Crawford, GE; Hatton, RD; Weaver, CT
      PLoS genetics  10  e1003969  2014

      Show Abstract
      24415943 24415943
      The molecular topography of silenced chromatin in Saccharomyces cerevisiae.
      Thurtle, DM; Rine, J
      Genes & development  28  245-58  2014

      Show Abstract
      24493645 24493645

      Posters

      Title
      Histone Modifications

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