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06-866 | Anti-acetyl-Histone H4 Antibody

200 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, T, Eu WB, ICC, ChIP Rb Serum Polyclonal Antibody
      Catalogue Number06-866
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H4 Antibody
      Alternate Names
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      Product Information
      • TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
      PresentationWhole antiserum containing 0.05% sodium azide.
      ApplicationUse Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) has been published and validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
      Epitopea.a. 2-19
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAcetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
      Species Reactivity
      • Human
      • Tetrahymena
      • Eukaryote
      Species Reactivity NoteHuman and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • IST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • 4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

      Western Blot Analysis:
      1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H4 (rabbit antiserum) - DAM1427455 DAM1427455
      Anti-acetyl-Histone H4 - ANTI-ACETYL-HISTONE
      Anti-acetyl-Histone H4 - 2384725 2384725
      Anti-acetyl-Histone H4 - 2459612 2459612
      Anti-acetyl-Histone H4 - 17428 17428
      Anti-acetyl-Histone H4 - 18784 18784
      Anti-acetyl-Histone H4 - 19336 19336
      Anti-acetyl-Histone H4 - 2019734 2019734
      Anti-acetyl-Histone H4 - 20667 20667
      Anti-acetyl-Histone H4 - 2073114 2073114
      Anti-acetyl-Histone H4 - 2153152 2153152
      Anti-acetyl-Histone H4 - 2282163 2282163
      Anti-acetyl-Histone H4 - 23201 23201
      Anti-acetyl-Histone H4 - 26393 26393
      Anti-acetyl-Histone H4 - 29532 29532
      Anti-acetyl-Histone H4 - 31992 31992
      Anti-acetyl-Histone H4 - DAM1416550 DAM1416550
      Anti-acetyl-Histone H4 - DAM1655258 DAM1655258
      Anti-acetyl-Histone H4 - DAM1690655 DAM1690655
      Anti-acetyl-Histone H4 - DAM1713199 DAM1713199
      Anti-acetyl-Histone H4 - DAM1739166 DAM1739166
      Anti-acetyl-Histone H4 - DAM1770301 DAM1770301
      Anti-acetyl-Histone H4 - DAM1834939 DAM1834939
      Anti-acetyl-Histone H4 - JBC1873473 JBC1873473
      Anti-acetyl-Histone H4 - JBC1885060 JBC1885060
      Anti-acetyl-Histone H4 -2554112 2554112
      Anti-acetyl-Histone H4 -2586451 2586451
      Anti-acetyl-Histone H4 -2605375 2605375
      Anti-acetyl-Histone H4 -2664262 2664262
      Anti-acetyl-Histone H4 -2717633 2717633
      Anti-acetyl-Histone H4 -2768452 2768452

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