07-352 Anti-acetyl-Histone H3 (Lys9) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt, Yeast (S. cerevisiae) WB, Mplex, ChIP-seq, ChIP Rb Serum Polyclonal Antibody
      Catalogue Number07-352
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys9) Antibody
      Alternate Names
      • H3K9Ac
      • Histone H3 (acetyl K9)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
      PresentationAntiserum containing 0.35% sodium azide and 30% glycerol.
      ApplicationAnti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H3 (Lys9) also known as H3K9Ac, Histone H3 (acetyl K9) and has been published and validated in ChIP, WB, Mplex.
      Key Applications
      • Western Blotting
      • Multiplexing
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation (ChIP):
      An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.

      Beadlyte ® Histone-Peptide Specificity Assay:
      A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No cross-reactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.
      Biological Information
      ImmunogenOvalbumin-conjugated, synthetic peptide (KQTARAcKSTGGK-C) corresponding to amino acids 4-14 of yeast histone H3 acetylated on lysine 9, with a C-terminal cysteine added for conjugation purposes.
      Epitopea.a. 4-14
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes acetyl-histone H3 (Lys9), Mr 17 kDa. An additional unknown protein was detected at Mr 23 kDa.
      Species Reactivity
      • Human
      • Vertebrates
      • Yeast (S. cerevisiae)
      Species Reactivity NoteYeast and human. Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on acid extracts from sodium butyrate treated HeLa cells.

      Western Blot Analysis:
      A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-acetyl-Histone H3 (Lys9) Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-acetyl-Histone H3 (Lys9) Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys9)2465196
      Anti-acetyl-Histone H3 (Lys9) - 23889342388934
      Anti-acetyl-Histone H3 (Lys9) - 20901522090152
      Anti-acetyl-Histone H3 (Lys9) - 22031262203126
      Anti-acetyl-Histone H3 (Lys9) - 2239922399
      Anti-acetyl-Histone H3 (Lys9) - 23250912325091
      Anti-acetyl-Histone H3 (Lys9) - 2466124661
      Anti-acetyl-Histone H3 (Lys9) - 2874128741
      Anti-acetyl-Histone H3 (Lys9) - 3138831388
      Anti-acetyl-Histone H3 (Lys9) - 31885953188595


      Reference overviewApplicationSpeciesPub Med ID
      Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels.
      Lorenzi, LE; Bah, A; Wischnewski, H; Shchepachev, V; Soneson, C; Santagostino, M; Azzalin, CM
      The EMBO journal  34  115-29  2015

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      The Epigenome of Schistosoma mansoni Provides Insight about How Cercariae Poise Transcription until Infection.
      Roquis, D; Lepesant, JM; Picard, MA; Freitag, M; Parrinello, H; Groth, M; Emans, R; Cosseau, C; Grunau, C
      PLoS neglected tropical diseases  9  e0003853  2015

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      The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection.
      Schliehe, C; Flynn, EK; Vilagos, B; Richson, U; Swaminathan, S; Bosnjak, B; Bauer, L; Kandasamy, RK; Griesshammer, IM; Kosack, L; Schmitz, F; Litvak, V; Sissons, J; Lercher, A; Bhattacharya, A; Khamina, K; Trivett, AL; Tessarollo, L; Mesteri, I; Hladik, A; Merkler, D; Kubicek, S; Knapp, S; Epstein, MM; Symer, DE; Aderem, A; Bergthaler, A
      Nature immunology  16  67-74  2015

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      Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects.
      Veazey, KJ; Parnell, SE; Miranda, RC; Golding, MC
      Epigenetics & chromatin  8  39  2015

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      Chromatin-Remodelling Complex NURF Is Essential for Differentiation of Adult Melanocyte Stem Cells.
      Koludrovic, D; Laurette, P; Strub, T; Keime, C; Le Coz, M; Coassolo, S; Mengus, G; Larue, L; Davidson, I
      PLoS genetics  11  e1005555  2015

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      Identification of and Molecular Basis for SIRT6 Loss-of-Function Point Mutations in Cancer.
      Kugel, S; Feldman, JL; Klein, MA; Silberman, DM; Sebastián, C; Mermel, C; Dobersch, S; Clark, AR; Getz, G; Denu, JM; Mostoslavsky, R
      Cell reports  13  479-88  2015

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      Genome wide mapping of UBF binding-sites in mouse and human cell lines.
      Diesch, J; Hannan, RD; Sanij, E
      Genomics data  3  103-5  2015

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      26484160 26484160
      Epigenetic mechanisms are involved in the regulation of ethanol consumption in mice.
      Qiang, M; Li, JG; Denny, AD; Yao, JM; Lieu, M; Zhang, K; Carreon, S
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

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      25522411 25522411
      Genome-wide targeting of the epigenetic regulatory protein CTCF to gene promoters by the transcription factor TFII-I.
      Peña-Hernández, R; Marques, M; Hilmi, K; Zhao, T; Saad, A; Alaoui-Jamali, MA; del Rincon, SV; Ashworth, T; Roy, AL; Emerson, BM; Witcher, M
      Proceedings of the National Academy of Sciences of the United States of America  112  E677-86  2015

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      Evaluation of the synuclein-γ (SNCG) gene as a PPARγ target in murine adipocytes, dorsal root ganglia somatosensory neurons, and human adipose tissue.
      Dunn, TN; Akiyama, T; Lee, HW; Kim, JB; Knotts, TA; Smith, SR; Sears, DD; Carstens, E; Adams, SH
      PloS one  10  e0115830  2015

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