07-539 Anti-acetyl-Histone H3 (Lys 4) Antibody

07-539
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H Mplex, WB, PIA, ChIP-seq, DB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-539
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys 4) Antibody
      Alternate Names
      • H3K4Ac
      • Histone H3 (acetyl K4)
      References
      Product Information
      FormatAffinity Purified
      Presentation100μl of immunoaffinity purified rabbit IgG in a buffer with 0.1M Tris-glycine, pH 7.4 and 0.15M NaCl, and containing 0.05% sodium azide and 30% glycerol
      Applications
      ApplicationAnti-acetyl-Histone H3 (Lys 4) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys 4) also known as H3K4Ac, Histone H3 (acetyl K4) & has been validated in Mplex, WB, PIA, ChIP, ChIP-seq, DB.
      Key Applications
      • Multiplexing
      • Western Blotting
      • Peptide Inhibition Assay
      • ChIP-seq
      • Dot Blot
      Application NotesSuggested dilutions:
      Western Blot: 1:1000 - 1:10,000
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...RT[AcK]Q... in which [AcK] corresponds to acetyl lysine 4 of human histone H3
      HostRabbit
      SpecificityHistone H3 when acetylated on Lys4
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteBroad species cross reactivity is expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      Modifications
      • Acetylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular WeightMr ~17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-acetyl-Histone H3 (Lys 4) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-acetyl-Histone H3 (Lys 4) Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys 4) - 2674726747
      Anti-acetyl-Histone H3 (Lys4) - 21160172116017
      Anti-acetyl-Histone H3 (Lys4) - 24304552430455
      Anti-acetyl-Histone H3 (Lys4) - 20441812044181
      Anti-acetyl-Histone H3 (Lys4) - 21683412168341
      Anti-acetyl-Histone H3 (Lys4) - 22778602277860
      Anti-acetyl-Histone H3 (Lys4) - 30467473046747
      Anti-acetyl-Histone H3 (Lys4) - 32006393200639
      Anti-acetyl-Histone H3 (Lys4) - JBC1900827JBC1900827
      Anti-acetyl-Histone H3 (Lys4) - NG1951652NG1951652

      References

      Reference overviewApplicationPub Med ID
      Hierarchical clustering of breast cancer methylomes revealed differentially methylated and expressed breast cancer genes.
      Lin, IH; Chen, DT; Chang, YF; Lee, YL; Su, CH; Cheng, C; Tsai, YC; Ng, SC; Chen, HT; Lee, MC; Chen, HW; Suen, SH; Chen, YC; Liu, TT; Chang, CH; Hsu, MT
      PloS one  10  e0118453  2015

      Show Abstract
      25706888 25706888
      Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane.
      Jeong, K; Kwon, H; Lee, J; Jang, D; Pak, Y
      Nucleic Acids Res  43  3114-27  2015

      Show Abstract
      25753664 25753664
      Dynamic chromatin modification sustains epithelial-mesenchymal transition following inducible expression of Snail-1.
      Javaid, S; Zhang, J; Anderssen, E; Black, JC; Wittner, BS; Tajima, K; Ting, DT; Smolen, GA; Zubrowski, M; Desai, R; Maheswaran, S; Ramaswamy, S; Whetstine, JR; Haber, DA
      Cell reports  5  1679-89  2013

      Show Abstract
      24360956 24360956
      Oral administration of the pimelic diphenylamide HDAC inhibitor HDACi 4b is unsuitable for chronic inhibition of HDAC activity in the CNS in vivo.
      Beconi, M; Aziz, O; Matthews, K; Moumné, L; O'Connell, C; Yates, D; Clifton, S; Pett, H; Vann, J; Crowley, L; Haughan, AF; Smith, DL; Woodman, B; Bates, GP; Brookfield, F; Bürli, RW; McAllister, G; Dominguez, C; Munoz-Sanjuan, I; Beaumont, V
      PloS one  7  e44498  2012

      Show Abstract
      Western Blotting22973455 22973455
      The new low-toxic histone deacetylase inhibitor S-(2) induces apoptosis in various acute myeloid leukemia cells.
      Cellai, C, et al.
      Journal of cellular and molecular medicine, (2011)  2011

      Show Abstract
      22004558 22004558
      H3 lysine 4 is acetylated at active gene promoters and is regulated by H3 lysine 4 methylation.
      Guillemette, B; Drogaris, P; Lin, HH; Armstrong, H; Hiragami-Hamada, K; Imhof, A; Bonneil, E; Thibault, P; Verreault, A; Festenstein, RJ
      PLoS genetics  7  e1001354  2011

      Show Abstract
      21483810 21483810
      A highly specific mechanism of histone H3-K4 recognition by histone demethylase LSD1
      Forneris, Federico, et al
      J Biol Chem, 281:35289-95 (2006)  2006

      16987819 16987819

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