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MABE224 | Anti-ZFX Antibody, clone 3E8.1

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABE224
      DescriptionAnti-ZFX Antibody, clone 3E8.1
      Alternate Names
      • Zinc finger X-chromosomal protein
      Background InformationZFX, also known as Zinc finger X-chromosomal protein, and encoded by the gene name ZFX, is probable transcriptional activator. This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined.
      Product Information
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-ZFX antibody is validated for use in WB, IC for the detection of ZFX.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected ZFX HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human ZFX.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZFX
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~130, ~90, ~59 kDa observed. Three bands (~130 kDa, ~91 kDa and ~59 kDa) corresponding to ZFX were detected. The ~130 kDa band is nuclear, while the ~91 kDa band was found in the cytoplasmic (Zhu, Z., et al. (2013). J Neurooncol. 112:17-25).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZFX in 200 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information