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05-776 | Anti-ZAP-70 Antibody, clone 1E7.2

05-776
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue Number05-776
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ZAP-70 Antibody, clone 1E7.2
      OverviewNote: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California
      References
      Product Information
      FormatPurified
      Presentationof 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-ZAP-70 Antibody, clone 1E7.2 is an antibody against ZAP-70 for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein between the second SH2 domain and precedes the kinase domain corresponding to residues 282-307 of human ZAP-70
      Clone1E7.2
      HostMouse
      SpecificityZap-70
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
      Gene Symbol
      • ZAP70
      • ZAP-70
      • TZK
      • STD
      • SRK
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
      SIZE: 619 amino acids; 69872 Da
      SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
      TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
      DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
      PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
      DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
      Molecular WeightMr ~70kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
      Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ZAP-70, clone 1E7.2 - 26824 26824
      Anti-ZAP-70, clone 1E7.2 - DAM1739159 DAM1739159
      Anti-ZAP-70, clone 1E7.2 - JBC1363175 JBC1363175
      Anti-Zap-70, clone 1E7.2 (mouse monoclonal IgG1) - 2464566 2464566
      Anti-Zap-70, clone 1E7.2 (mouse monoclonal IgG1) - 2187218 2187218
      Anti-Zap-70, clone 1E7.2 (mouse monoclonal IgG1) - 2273888 2273888
      Anti-Zap-70, clone 1E7.2 -2521194 2521194

      References

      Reference overviewPub Med ID
      TCR signaling: another Abl-bodied kinase joins the cascade.
      Wange, Ronald L
      Curr. Biol., 14: R562-4 (2004) 2004

      Show Abstract
      15268875 15268875

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      Included Positive Control

      Catalog Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies