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MABE1123 | Anti-XPB Antibody, clone 15TF2-1B3

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Ascites Monoclonal Antibody
      Catalogue NumberMABE1123
      DescriptionAnti-XPB Antibody, clone 15TF2-1B3
      Alternate Names
      • TFIIH basal transcription factor complex helicase XPB subunit
      • Basic transcription factor 2 89 kDa subunit
      • BTF2 p89
      • DNA excision repair protein ERCC-3
      • DNA repair protein complementing XP-B cells
      • TFIIH basal transcription factor complex 89 kDa subunit
      • TFIIH 89 kDa subunit
      • TFIIH p89
      • Xeroderma pigmentosum group B-complementing protein
      • XPB
      Background InformationTranscription factor II human (TFIIH) basal transcription factor complex helicase XPB subunit (EC; UniProt P19447; also known as BTF2 p89, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH p89, Basic transcription factor 2 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein) is encoded by the ERCC3 (also known as BTF2, GTF2H, RAD25, TFIIH, XPB) gene (Gene ID 2071) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by TFIIH. The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.
      Product Information
      PresentationMouse monoclonal IgG1κ ascites with 0.05% sodium azide.
      ApplicationThis Anti-XPB Antibody, clone 15TF2-1B3 is validated for use in Western Blotting, Immunocytochemistry for the detection of XPB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Xpb in murine embryonic fibroblasts (MEFs) and HeLa cells, as well as in transgenic animal-derived MEFs expressing Xpb lacking last 43 C-terminal amino acids (Andressoo, J.O., et al. (2009). Mol Cell Biol.29(5):1276-290).
      Western Blotting Analysis: A representative lot detected endougenous as well as exogenously expressed Xpb in both U2OS17 whole cell lysate and in THIIF p62 subunit immunoprecipitate (Ziani, S., et al. (2014). J Cell Biol.;206(5):589-598).
      Western Blotting Analysis: A representative lot detected Xpb in THIIF TTDA subunit immunoprecipitate (Giglia-Mari,G., et al. (2006). PLoS Biol. 4(6): e156).
      Immunocytochemistry Analysis: A representative lot detected XPB recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).
      Biological Information
      ImmunogenRecombinant protein corresponding to human XPB.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ERCC3
      • XPB
      • XPBC
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~85 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPB in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-XPB, clone 15TF2-1B3 -Q2589558 Q2589558


      Reference overviewPub Med ID
      A small molecule screen identifies an inhibitor of DNA repair inducing the degradation of TFIIH and the chemosensitization of tumor cells to platinum.
      Alekseev, S; Ayadi, M; Brino, L; Egly, JM; Larsen, AK; Coin, F
      Chemistry & biology 21 398-407 2014

      Show Abstract
      24508195 24508195
      Sequential and ordered assembly of a large DNA repair complex on undamaged chromatin.
      Ziani, S; Nagy, Z; Alekseev, S; Soutoglou, E; Egly, JM; Coin, F
      The Journal of cell biology 206 589-98 2014

      Show Abstract
      25154395 25154395
      An Xpb mouse model for combined xeroderma pigmentosum and cockayne syndrome reveals progeroid features upon further attenuation of DNA repair.
      Andressoo, JO; Weeda, G; de Wit, J; Mitchell, JR; Beems, RB; van Steeg, H; van der Horst, GT; Hoeijmakers, JH
      Molecular and cellular biology 29 1276-90 2009

      Show Abstract
      19114557 19114557
      Dynamic interaction of TTDA with TFIIH is stabilized by nucleotide excision repair in living cells.
      Giglia-Mari, G; Miquel, C; Theil, AF; Mari, PO; Hoogstraten, D; Ng, JM; Dinant, C; Hoeijmakers, JH; Vermeulen, W
      PLoS biology 4 e156 2006

      Show Abstract
      16669699 16669699

      Technical Info

      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System