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ABT79 | Anti-WASH complex subunit FAM21C Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC Rb Purified Polyclonal Antibody
      Catalogue NumberABT79
      DescriptionAnti-WASH complex subunit FAM21C Antibody
      Alternate Names
      • family with sequence similarity 21, member C
      • vaccinia virus penetration factor
      • WASH complex subunit FAM21C
      Background InformationFAM21C, also known as WASH complex subunit FAM21C, is a part of the a 500 kDa core complex that allows the Wiskott-Aldrich syndrome protein (WASH) function. FAM21C can be found in the membranes of early endosomes. FAM21C cooperates with CAPZ and hinders its actin-capping activity, links WASH to endosomes, and is required for WASH-dependent retromer-mediated sorting.
      Product Information
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-WASH complex subunit FAM21C Antibody detects level of WASH complex subunit FAM21C & has been published & validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilutioin from a previous lot detected WASH complex subunit FAM21C in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human WASH complex subunit FAM21C.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FAM21C
      • FAM21A
      • VPEF2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes.

      SUBUNIT STRUCTURE: Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via N-terminus) with WASH1; the interaction is direct.

      SUBCELLULAR LOCATION: Early endosome membrane

      MISCELLANEOUS: In human, FAM21 has undergone evolutionary duplication, with 4 highly homologous family members existing, including FAM21A, FAM21B, FAM21C, and an N-terminally truncated FAM21D form.

      SEQUENCE SIMILARITIES: Belongs to the FAM21 family.

      SEQUENCE CAUTION: The sequence AAH06456.1 differs from that shown. Reason: Erroneous initiation.

      The sequence BAA25518.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight ~145 kDa observed
      Non-specific bands may be observed >200 kDa and at ~10 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit FAM21C in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-WASH complex subunit FAM21C - 2140074 2140074
      Anti-WASH complex subunit FAM21C - 2424801 2424801
      Anti-WASH complex subunit FAM21C - 2438675 2438675
      Anti-WASH complex subunit FAM21C - 1951091 1951091
      Anti-WASH complex subunit FAM21C - 2211056 2211056
      Anti-WASH complex subunit FAM21C - 2301425 2301425
      Anti-WASH complex subunit FAM21C - NRG1832112 NRG1832112
      Anti-WASH complex subunit FAM21C -2688592 2688592
      Anti-WASH complex subunit FAM21C -2733676 2733676
      Anti-WASH complex subunit FAM21C -2768410 2768410