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CBL202 | Anti-Vimentin Antibody, clone VIM 3B4

CBL202
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Am, B, Ca, Ch, H, Mk ELISA, IF, IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberCBL202
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vimentin Antibody, clone VIM 3B4
      References
      Product Information
      FormatPurified
      Control
      • Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
      PresentationProtein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
      Applications
      ApplicationThis Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern blot
      Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
      Immunofluorescence
      ELISA

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenVimentin purified from bovine lens
      CloneVIM 3B4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHighly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
      IsotypeIgG2a
      Species Reactivity
      • Amphibian
      • Bovine
      • Canine
      • Chicken
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlong with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
      Gene Symbol
      • VIM
      • FLJ36605
      • Vimentin.
      • vimentin
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
      SIZE: 466 amino acids; 53652 Da
      SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
      TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
      PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
      SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.
      Molecular Weight57 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2–8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN VIMENTIN 2465136
      MOUSE ANTI-HUMAN VIMENTIN MONOCLONAL ANTIBODY - 2377348 2377348
      MOUSE ANTI-HUMAN VIMENTIN -2746516 2746516
      MOUSE ANTI-HUMAN VIMENTIN -2799132 2799132
      MOUSE ANTI-HUMAN VIMENTIN MONOCLONAL ANTIBODY - 2063134 2063134
      MOUSE ANTI-HUMAN VIMENTIN MONOCLONAL ANTIBODY - 2227091 2227091
      MOUSE ANTI-HUMAN VIMENTIN MONOCLONAL ANTIBODY - 2325049 2325049

      References | 17 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
      Pereyra-Bonnet, F; Gimeno, ML; Argumedo, NR; Ielpi, M; Cardozo, JA; Giménez, CA; Hyon, SH; Balzaretti, M; Loresi, M; Fainstein-Day, P; Litwak, LE; Argibay, PF
      PloS one 9 e100369 2014

      Show Abstract
      24963634 24963634
      Elevated Orai1 expression mediates tumor-promoting intracellular Ca2+ oscillations in human esophageal squamous cell carcinoma.
      Zhu, H; Zhang, H; Jin, F; Fang, M; Huang, M; Yang, CS; Chen, T; Fu, L; Pan, Z
      Oncotarget 5 3455-71 2014

      Show Abstract
      24797725 24797725
      Dynamic expression and localization of c-MET isoforms in the developing rat pancreas.
      Wu, Y; Cheng, M; Shi, Z; Feng, Z; Guan, X
      International journal of clinical and experimental pathology 7 8563-72 2014

      Show Abstract
      25674220 25674220
      Recrudescence mechanisms and gene expression profile of the reproductive tracts from chickens during the molting period.
      Jeong, W; Lim, W; Ahn, SE; Lim, CH; Lee, JY; Bae, SM; Kim, J; Bazer, FW; Song, G
      PloS one 8 e76784 2013

      Show Abstract
      ImmunohistochemistryChicken24098561 24098561
      Dynamic epigenetic regulation of the microRNA-200 family mediates epithelial and mesenchymal transitions in human tumorigenesis.
      V Davalos,C Moutinho,A Villanueva,R Boque,P Silva,F Carneiro,M Esteller
      Oncogene 31 2012

      Show Abstract
      21874049 21874049
      Sites of origin and developmental dynamics of the neurons in the core and shell regions of torus semicircularis in the Chinese softshell turtle (Pelodiscus sinensis).
      Chao Xi,Qiong Chen,Shao-Ju Zeng,Yu-Tao Lin,Yu-Fang Huang,Yu Liu,Xin-Wen Zhang,Ming-Xue Zuo
      The Journal of comparative neurology 519 2011

      Show Abstract
      21484802 21484802
      α-fetoprotein involvement during glucocorticoid-induced precocious maturation in rat colon.
      Chen M, Sun P, Liu XY, Dong D, Du J, Gu L, Ge YB
      World journal of gastroenterology : WJG 17 2933-40. 2011

      Full Text Article
      21734804 21734804
      Expression and location of alpha-fetoprotein during rat colon development.
      Xiao-Yan Liu, Dan Dong, Peng Sun, Jun Du, Luo Gu, Ying-Bin Ge, Xiao-Yan Liu, Dan Dong, Peng Sun, Jun Du, Luo Gu, Ying-Bin Ge, Xiao-Yan Liu, Dan Dong, Peng Sun, Jun Du, Luo Gu, Ying-Bin Ge, Xiao-Yan Liu, Dan Dong, Peng Sun, Jun Du, Luo Gu, Ying-Bin Ge
      World journal of gastroenterology : WJG 15 1738-43 2009

      Show Abstract Full Text Article
      19360917 19360917
      Phenotypic plasticity of neoplastic ovarian epithelium: unique cadherin profiles in tumor progression.
      Hudson, LG; Zeineldin, R; Stack, MS
      Clinical & experimental metastasis 25 643-55 2008

      Show Abstract Full Text Article
      18398687 18398687
      Expression and localization of mesothelin in developing rat pancreas.
      Liang-Qin Hou, Yin-He Wang, Li-Jie Liu, Jing Guo, Li-Ping Teng, Li-Hua Cao, Hui Shi, Li Yuan, Wei De, Liang-Qin Hou, Yin-He Wang, Li-Jie Liu, Jing Guo, Li-Ping Teng, Li-Hua Cao, Hui Shi, Li Yuan, Wei De, Liang-Qin Hou, Yin-He Wang, Li-Jie Liu, Jing Guo, Li-Ping Teng, Li-Hua Cao, Hui Shi, Li Yuan, Wei De
      Development, growth differentiation 50 531-41 2008

      Show Abstract
      18505465 18505465

      Data Sheet

      Title
      MOUSE ANTI-HUMAN VIMENTIN

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies