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MAB3400 | Anti-Vimentin Antibody, clone V9

40 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, Ch, Fe, H, Mk, Po, R ICC, IHC, IH(P), WB M Purified Monoclonal Antibody
      Catalogue NumberMAB3400
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vimentin Antibody, clone V9
      Background InformationVimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
      Product Information
      • Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter
      PresentationPurified mouse monoclonal IgG1 presented in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
      ApplicationAnti-Vimentin Antibody, clone V9 is an antibody against Vimentin for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunoblotting:
      A dilution of 1:1000 was used in a previous lot.

      5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.

      5-10 μg/mL of a previous lot was used in IC.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified vimentin from pig eye lens
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Feline
      • Human
      • Monkey
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlong with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
      Gene Symbol
      • VIM
      • FLJ36605
      Non-Reactive Species
      • Mouse
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
      SIZE: 466 amino acids; 53652 Da
      SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
      TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
      PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
      SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family. ...
      Molecular Weight57-60 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on HeLa lysates.

      Western Blot:
      1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size40 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Vimentin, clone V9 - 2373398 2373398
      Anti-Vimentin, clone V9 - 1945579 1945579
      Anti-Vimentin, clone V9 - 1983686 1983686
      Anti-Vimentin, clone V9 - 2062311 2062311
      Anti-Vimentin, clone V9 - 2216878 2216878
      Anti-Vimentin, clone V9 - LV1506369 LV1506369
      Anti-Vimentin, clone V9 - LV1594097 LV1594097
      Anti-Vimentin, clone V9 - LV1700725 LV1700725
      Anti-Vimentin, clone V9 - LV1777812 LV1777812
      Anti-Vimentin, clone V9 - NG1878288 NG1878288
      Anti-Vimentin, clone V9 -2488952 2488952
      Anti-Vimentin, clone V9 -2515015 2515015
      Anti-Vimentin, clone V9 -2570217 2570217
      Anti-Vimentin, clone V9 -2583352 2583352
      Anti-Vimentin, clone V9 -2586466 2586466
      Anti-Vimentin, clone V9 -2618486 2618486
      Anti-Vimentin, clone V9 -2664464 2664464
      Anti-Vimentin, clone V9 -2710697 2710697
      Anti-Vimentin, clone V9 -2762323 2762323

      References | 39 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Platelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells.
      Orellana, R; Kato, S; Erices, R; Bravo, ML; Gonzalez, P; Oliva, B; Cubillos, S; Valdivia, A; Ibañez, C; Brañes, J; Barriga, MI; Bravo, E; Alonso, C; Bustamente, E; Castellon, E; Hidalgo, P; Trigo, C; Panes, O; Pereira, J; Mezzano, D; Cuello, MA; Owen, GI
      BMC cancer 15 290 2015

      Show Abstract
      25886038 25886038
      Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model.
      Ishikawa, M; Yoshitomi, T; Zorumski, CF; Izumi, Y
      Investigative ophthalmology & visual science 55 8531-41 2014

      Show Abstract
      25406290 25406290
      An image-based, dual fluorescence reporter assay to evaluate the efficacy of shRNA for gene silencing at the single-cell level.
      Kojima, S; Borisy, GG
      F1000Research 3 60 2014

      Show Abstract
      Western Blotting24741441 24741441
      Distal renal tubules are deficient in aggresome formation and autophagy upon aldosterone administration.
      Cheema, MU; Damkier, HH; Nielsen, J; Poulsen, ET; Enghild, JJ; Fenton, RA; Praetorius, J
      PloS one 9 e101258 2014

      Show Abstract
      25000288 25000288
      Misfolded polyglutamine, polyalanine, and superoxide dismutase 1 aggregate via distinct pathways in the cell.
      Polling, S; Mok, YF; Ramdzan, YM; Turner, BJ; Yerbury, JJ; Hill, AF; Hatters, DM
      The Journal of biological chemistry 289 6669-80 2014

      Show Abstract
      24425868 24425868
      Repair of astrocytes, blood vessels, and myelin in the injured brain: possible roles of blood monocytes.
      Jeong, HK; Ji, KM; Kim, J; Jou, I; Joe, EH
      Molecular brain 6 28 2013

      Show Abstract
      23758980 23758980
      Improving outcomes of acute kidney injury using mouse renal progenitor cells alone or in combination with erythropoietin or suramin.
      Han, X; Zhao, L; Lu, G; Ge, J; Zhao, Y; Zu, S; Yuan, M; Liu, Y; Kong, F; Xiao, Z; Zhao, S
      Stem cell research & therapy 4 74 2013

      Show Abstract
      23777889 23777889
      Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules.
      Cheema, MU; Poulsen, ET; Enghild, JJ; Hoorn, EJ; Hoorn, E; Fenton, RA; Praetorius, J
      Physiological reports 1 e00064 2013

      Show Abstract
      24303148 24303148
      Survival of cancer stem cells under hypoxia and serum depletion via decrease in PP2A activity and activation of p38-MAPKAPK2-Hsp27.
      Lin, SP; Lee, YT; Wang, JY; Miller, SA; Chiou, SH; Hung, MC; Hung, SC
      PloS one 7 e49605 2012

      Show Abstract
      Western Blotting23185379 23185379
      Expression of tissue transglutaminase on primary olfactory ensheathing cells cultures exposed to stress conditions.
      Agata Campisi,Michela Spatuzza,Antonella Russo,Giuseppina Raciti,Angelo Vanella,Stefania Stanzani,Rosalia Pellitteri
      Neuroscience research 72 2012

      Show Abstract
      22222252 22222252

      Data Sheet

      Anti-Vimentin, clone V9 - Data Sheet
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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