AB5905 Anti-Vesicular Glutamate Transporter 1 Antibody

AB5905
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R IHC Gp Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5905
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vesicular Glutamate Transporter 1 Antibody
      Alternate Names
      • VGLUT1
      • BNPI
      Background InformationVGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.
      References
      Product Information
      FormatSerum
      Control
      • Rat brain lysate
      PresentationGuinea pig Serum in 0.1% Sodium Azide.
      Applications
      ApplicationThis Anti-Vesicular Glutamate Transporter 1 Antibody is validated for use in Immunohistochemistry for the detection of Vesicular Glutamate Transporter 1 with more than 55 product citations.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:5,000-1:10,000 using a cyanine conjugated secondary antibody.

      Lightly fixed 4% PFA fixed materials are recommended. Enzymatic detection requires substantially higher primary antibody dilutions.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide from rat VGLUT1 protein with no overlap to VGLUT2. The immunogen is available as AG208.
      ConcentrationVariable
      HostGuinea Pig
      SpecificityRecognizes Vesicular Glutamate Transporter 1 (VGLUT1, BNPI {Brain specific Na+ dependent inorganic phosphate cotransporter}). The antiserum has been tested on tissue sections from the rat central nervous system (CNS) using immunofluorescence histochemistry. The antiserum mainly labels nerve fibers and terminals. VGLUT1 is an excellent marker for glutamatergic nerve terminals. The staining pattern for the staining obtained with the VGLUT1 antiserum corresponds to the pattern described using other antisera to VGLUT1 (Bellocchio et al., 1998; Fremeau et al., 2001; Fujiyama et al., 2001; Sakata-Haga et al., 2001; Tong et al., 2001; Kaneko et al. 2002; Varoqui et al., 2002). Preabsorption of the VGLUT1 antiserum with immunogen peptide eliminates all immunostaining.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summarya Na(+)-dependent inorganic phosphate cotransporter.
      Gene Symbol
      • VGluT1
      • Solute carrier family 17 member 7
      • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
      • Slc17a7
      • Bnpi
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
      SIZE: 560 amino acids; 61665 Da [MW of unprocessed precursor]
      SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome.
      TISSUE SPECIFICITY: Expressed in the cerebellum, cerberal cortex and hippocampus. Isoform 2 is expressed specifically in retina.
      SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
      Molecular Weight62 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-VGLUT1 - 23707332370733
      Anti-VGLUT1 - 23875282387528
      Anti-VGLUT1 - 24304982430498
      Anti-VGLUT1 - 24556442455644
      Anti-VGLUT1 - 31938443193844
      Anti-VGLUT1 -24842432484243
      Anti-VGLUT1 -25720592572059
      Anti-VGLUT1 -26033082603308
      Anti-VGLUT1 -26475292647529
      Anti-VGLUT1 -26519192651919

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cellular plasticity induced by anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies.
      Peng, X; Hughes, EG; Moscato, EH; Parsons, TD; Dalmau, J; Balice-Gordon, RJ
      Annals of neurology  77  381-98  2015

      Show Abstract
      25369168 25369168
      Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5.
      Brigidi, GS; Santyr, B; Shimell, J; Jovellar, B; Bamji, SX
      Nature communications  6  8200  2015

      Show Abstract
      26334723 26334723
      Aberrant synaptic integration in adult lamina I projection neurons following neonatal tissue damage.
      Li, J; Kritzer, E; Craig, PE; Baccei, ML
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  2438-51  2015

      Show Abstract
      25673839 25673839
      Loss of δ-catenin function in severe autism.
      Turner, TN; Sharma, K; Oh, EC; Liu, YP; Collins, RL; Sosa, MX; Auer, DR; Brand, H; Sanders, SJ; Moreno-De-Luca, D; Pihur, V; Plona, T; Pike, K; Soppet, DR; Smith, MW; Cheung, SW; Martin, CL; State, MW; Talkowski, ME; Cook, E; Huganir, R; Katsanis, N; Chakravarti, A
      Nature  520  51-6  2015

      Show Abstract
      25807484 25807484
      Leucine-rich repeat kinase 2 modulates neuroinflammation and neurotoxicity in models of human immunodeficiency virus 1-associated neurocognitive disorders.
      Puccini, JM; Marker, DF; Fitzgerald, T; Barbieri, J; Kim, CS; Miller-Rhodes, P; Lu, SM; Dewhurst, S; Gelbard, HA
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  5271-83  2015

      Show Abstract
      25834052 25834052
      Cis and trans RET signaling control the survival and central projection growth of rapidly adapting mechanoreceptors.
      Fleming, MS; Vysochan, A; Paixão, S; Niu, J; Klein, R; Savitt, JM; Luo, W
      eLife  4  e06828  2015

      Show Abstract
      25838128 25838128
      Mapping synapses by conjugate light-electron array tomography.
      Collman, F; Buchanan, J; Phend, KD; Micheva, KD; Weinberg, RJ; Smith, SJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  5792-807  2015

      Show Abstract
      25855189 25855189
      Loss of F-box only protein 2 (Fbxo2) disrupts levels and localization of select NMDA receptor subunits, and promotes aberrant synaptic connectivity.
      Atkin, G; Moore, S; Lu, Y; Nelson, RF; Tipper, N; Rajpal, G; Hunt, J; Tennant, W; Hell, JW; Murphy, GG; Paulson, H
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  6165-78  2015

      Show Abstract
      25878288 25878288
      Adolescent intermittent alcohol exposure: persistence of structural and functional hippocampal abnormalities into adulthood.
      Risher, ML; Fleming, RL; Risher, WC; Miller, KM; Klein, RC; Wills, T; Acheson, SK; Moore, SD; Wilson, WA; Eroglu, C; Swartzwelder, HS
      Alcoholism, clinical and experimental research  39  989-97  2015

      Show Abstract
      25916839 25916839
      Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture.
      Paşca, AM; Sloan, SA; Clarke, LE; Tian, Y; Makinson, CD; Huber, N; Kim, CH; Park, JY; O'Rourke, NA; Nguyen, KD; Smith, SJ; Huguenard, JR; Geschwind, DH; Barres, BA; Paşca, SP
      Nature methods  12  671-8  2015

      Show Abstract
      26005811 26005811

      Data Sheet

      Title
      Anti-Vesicular Glutamate Transporter 1 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies