AB1588 Anti-Vesicular Acetylcholine Transporter Antibody

AB1588
20 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R IHC, WB Gp Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1588
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vesicular Acetylcholine Transporter Antibody
      Alternate Names
      • VAChT
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Lyophilized from 0.1 M PBS with 1.0% BSA and 0.1% sodium azide. Reconstitute with 100 μL of sterile distilled water.
      Applications
      ApplicationThis Anti-Vesicular Acetylcholine Transporter Antibody is validated for use in IH, WB for the detection of Vesicular Acetylcholine Transporter.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry: 1:500-1:1,500 on 4% paraformaldehyde fixed tissue. Sections were cut at 15 μm with a cryostat or 30 μm with a sliding microtome.

      Immunoblotting

      Blocking peptide available (Catalog Number AG223).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminus of the predicted rat VAChT protein.
      HostGuinea Pig
      SpecificityVesicular Acetylcholine Transporter (VAChT). By Western blot the antibody reacted with a single band in rat brain homogenates. This band corresponded to the size of the predicted rVAChT. There was no staining of blots incubated with the antibody that had been preabsorbed with the peptide used for antibody production. By immunohistochemistry the staining was strong in the inner retina where cholinergic processes are located and weak in cholinergic cell bodies. There was no background staining noted. In the CNS staining was strong in fibers and stains cholinergic cell bodies well.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
      Gene Symbol
      • SLC18A3
      • MGC12716
      • VACHT
      • VAChT
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16572 # Involved in acetylcholine transport into synaptic vesicles.
      SIZE: 532 amino acids; 56961 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Peripheral and central cholinergic nervous systems.
      SIMILARITY: SwissProt: Q16572 ## Belongs to the major facilitator superfamily. Vesicular transporter family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at 2-8°C for up to 12 months. Maintain reconstituted material at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 23706712370671
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 23909892390989
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER -24887732488773
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 20027112002711
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 21671222167122
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 22243882224388
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 23435552343555

      References

      Reference overviewApplicationSpeciesPub Med ID
      A major external source of cholinergic innervation of the striatum and nucleus accumbens originates in the brainstem.
      Dautan, D; Huerta-Ocampo, I; Witten, IB; Deisseroth, K; Bolam, JP; Gerdjikov, T; Mena-Segovia, J
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  4509-18  2014

      Show Abstract
      Immunohistochemistry24671996 24671996
      Isl1 directly controls a cholinergic neuronal identity in the developing forebrain and spinal cord by forming cell type-specific complexes.
      Cho, HH; Cargnin, F; Kim, Y; Lee, B; Kwon, RJ; Nam, H; Shen, R; Barnes, AP; Lee, JW; Lee, S; Lee, SK
      PLoS genetics  10  e1004280  2014

      Show Abstract
      Immunohistochemistry24763339 24763339
      Motor axon synapses on renshaw cells contain higher levels of aspartate than glutamate.
      Richards, DS; Griffith, RW; Romer, SH; Alvarez, FJ
      PloS one  9  e97240  2014

      Show Abstract
      24816812 24816812
      Severe drug-induced repetitive behaviors and striatal overexpression of VAChT in ChAT-ChR2-EYFP BAC transgenic mice.
      Crittenden, JR; Lacey, CJ; Lee, T; Bowden, HA; Graybiel, AM
      Frontiers in neural circuits  8  57  2014

      Show Abstract
      24904300 24904300
      Genetic dissection of retinal inputs to brainstem nuclei controlling image stabilization.
      Dhande, OS; Estevez, ME; Quattrochi, LE; El-Danaf, RN; Nguyen, PL; Berson, DM; Huberman, AD
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  17797-813  2013

      Show Abstract
      Immunohistochemistry24198370 24198370
      B6eGFPChAT mice overexpressing the vesicular acetylcholine transporter exhibit spontaneous hypoactivity and enhanced exploration in novel environments.
      Nagy, PM; Aubert, I
      Brain and behavior  3  367-83  2013

      Show Abstract
      Western BlottingMouse24381809 24381809
      Transsynaptic tracing with vesicular stomatitis virus reveals novel retinal circuitry.
      Beier, KT; Borghuis, BG; El-Danaf, RN; Huberman, AD; Demb, JB; Cepko, CL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  35-51  2013

      Show Abstract
      23283320 23283320
      Caudo-rostral brain spreading of α-synuclein through vagal connections.
      Ulusoy, A; Rusconi, R; Pérez-Revuelta, BI; Musgrove, RE; Helwig, M; Winzen-Reichert, B; Di Monte, DA
      EMBO molecular medicine  5  1051-9  2013

      Show Abstract
      Immunohistochemistry23703938 23703938
      Alterations in the motor neuron-renshaw cell circuit in the Sod1(G93A) mouse model.
      Wootz, H; Fitzsimons-Kantamneni, E; Larhammar, M; Rotterman, TM; Enjin, A; Patra, K; André, E; Van Zundert, B; Kullander, K; Alvarez, FJ
      The Journal of comparative neurology  521  1449-69  2013

      Show Abstract
      23172249 23172249
      EGFR inhibitor erlotinib delays disease progression but does not extend survival in the SOD1 mouse model of ALS.
      Le Pichon, CE; Dominguez, SL; Solanoy, H; Ngu, H; Lewin-Koh, N; Chen, M; Eastham-Anderson, J; Watts, R; Scearce-Levie, K
      PloS one  8  e62342  2013

      Show Abstract
      ImmunohistochemistryMouse23638043 23638043

      Data Sheet

      Title
      GUNIEA PIG ANTI-VESICULAR ACETYLCHOLINE TRANSPORTER