A part of MilliporeSigma

ABN100 | Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M IHC, ELISA Gt Affinity Purified Polyclonal Antibody
      Catalogue NumberABN100
      DescriptionAnti-Vesicular Acetylcholine Transporter (VAChT) Antibody
      Alternate Names
      • Vesicular acetylcholine transporter
      • VAChT
      • rVAT
      • Solute carrier family 18 member 3
      Background InformationAcetylcholine mediates cholinergic transmission in the central and peripheral nervous systems, and is an important modulator of cognition, movement, and cardiovascular function, among other processes. The effects of acetylcholine are regulated by its synthesis, storage, and breakdown, which involve the related enzymes choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT), and acetylcholinesterase (AChE), respectively. VAChT is primarily found in the membrane of presynaptic secretory vesicles. It contains 12 transmembrane domains and a synaptic-vesicular–targeting signal located on the intracellular C-terminal tail. The primary function of VAChT is to actively transport cytoplasmic ACh, across an electrochemical gradient, into synaptic vesicles that release ACh upon cell stimulation. The VAChT can affect cholinergic signaling by affecting the relative pools of ACh secreted to activate postsynaptic nicotinic and muscarinic receptors. VAChT is phosphorylated on its serine 480 residue in the C-terminal tail by protein kinase C. Although the precise role of this modification is still unclear, previous studies suggest that it may affect the localization of VAChT to synaptic vesicles.
      Product Information
      FormatAffinity Purified
      • Vesicular Acetylcholine Transporter (VAChT) peptide
      PresentationPurified goat polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Vesicular Acetylcholine Transporter (VAChT) Antibody detects level of Vesicular Acetylcholine Transporter (VAChT) & has been published & validated for use in IHC, ELISA.
      Key Applications
      • Immunohistochemistry
      • ELISA
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Acetylcholine Transporter (VAChT) in rat midbrain tissue.
      ELISA Analysis: A representative lot detected Vesicular Acetylcholine Transporter (VAChT) in a direct ELISA using a corresponding peptide.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of rat VAChT.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the C-terminus of Vesicular Acetylcholine Transporter (VAChT).
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with Rat and Mouse. Other homologies: Human (95% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc18a3
      • Vacht
      • Vat
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight57 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry Analysis.
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Acetylcholine Transporter (VAChT) in mouse midbrain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Vesicular Acetylcholine -2707197 2707197
      Anti-Vesicular Acetylcholine -2709035 2709035
      Anti-Vesicular Acetylcholine -2778849 2778849
      Anti-Vesicular Acetylcholine -2819596 2819596
      Anti-Vesicular Acetylcholine Transporter (VAChT) 2465302
      Anti-Vesicular Acetylcholine Transporter (VAChT) - 2388359 2388359
      Anti-Vesicular Acetylcholine Transporter (VAChT) - 2438664 2438664
      Anti-Vesicular Acetylcholine Transporter (VAChT) - 2199694 2199694
      Anti-Vesicular Acetylcholine Transporter (VAChT) - 2294373 2294373
      Anti-Vesicular Acetylcholine Transporter (VAChT) - 2562015 2562015
      Anti-Vesicular Acetylcholine Transporter (VAChT) - Q2064443 Q2064443
      Anti-Vesicular Acetylcholine Transporter (VAChT) -2514258 2514258
      Anti-Vesicular Acetylcholine Transporter (VAChT) -2564876 2564876
      Anti-Vesicular Acetylcholine Transporter (VAChT) -2615718 2615718
      Anti-Vesicular Acetylcholine Transporter (VAChT) -2742260 2742260


      Reference overviewPub Med ID
      Endogenous cholinergic neurotransmission contributes to behavioral sensitization to morphine.
      Bajic, D; Soiza-Reilly, M; Spalding, AL; Berde, CB; Commons, KG
      PloS one 10 e0117601 2015

      Show Abstract
      25647082 25647082
      Forebrain deletion of the dystonia protein torsinA causes dystonic-like movements and loss of striatal cholinergic neurons.
      Pappas, SS; Darr, K; Holley, SM; Cepeda, C; Mabrouk, OS; Wong, JM; LeWitt, TM; Paudel, R; Houlden, H; Kennedy, RT; Levine, MS; Dauer, WT
      eLife 4 e08352 2015

      Show Abstract
      26052670 26052670
      A genetically specified connectomics approach applied to long-range feeding regulatory circuits.
      Atasoy, D; Betley, JN; Li, WP; Su, HH; Sertel, SM; Scheffer, LK; Simpson, JH; Fetter, RD; Sternson, SM
      Nature neuroscience 17 1830-9 2014

      Show Abstract
      25362474 25362474
      Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain.
      Vetreno, RP; Broadwater, M; Liu, W; Spear, LP; Crews, FT
      PloS one 9 e113421 2014

      Show Abstract
      25405505 25405505

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      Life Science Research > Antibodies and Assays > Primary Antibodies