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MAB3757 | Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9

MAB3757
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, IF, IH(P), IP, WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB3757
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9
      Alternate Names
      • VEGFR3
      • VEGFR-3
      • Receptor tyrosine Kinase VEGFR-3
      Background InformationVascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. {Bando, 2004}. Jussila et al reported that VEGFR3 antibodies specifically stain endothelial cells of lymphatic vessels and vessels around tumonrs such as lymphoma and in situ breast carcinoma. {Jussila, 1998}.Interestingly, the spindle cells of several cutaneous nodular AIDS-associated Kaposi's sarcomas and the endothelium around the nodules were also VEGFR-3 positive. The first specific molecular marker for the lymphatic endothelium should provide a useful tool for the analysis of lymphatic vessels in malignant tumors and their metastases and the cellular origin and differentiation of Kaposi's sarcomas. {Jussila, 1998}.
      References
      Product Information
      FormatAscites
      Control
      • Human lymphatic tissues
      • VEGFR-3 is not expressed in HEVs of the lymph nodes. {Jussila, 1998]. In lymphomas staining is seen mostly in collapsed vessels in the cortex of lymph nodes infiltrated by the lymphoma cells. The antibody specifically detects lymphatic endothelium in human lymph node and other organs such as tonsil. See Jussila et al, 1998 for complete tissue characterization.
      PresentationUnPurified ascites fluid containing 0.1% sodium azide as a preservative.
      Applications
      ApplicationAnti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9 is an antibody against VEGF Receptor-3 for use in FC, IF, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry: frozen cryosections, 5μm cryosections were air-dried and fixed in cold acetone for 10 minutes. Blocked with 5% normal horse serum and then incubated with 9D9 for 2 hours at room temperature in a humid chamber. Typical dilutions will range from 1:100-1:1000 depending upon lot titer. Detection is with enhanced methods (ABC or poly HRP) and enzymatic substrates for best results.

      Immunohistochemistry in paraffin: 5μm thick lightly fixed formalin tissues were processed through decreasing ethanol concentrations, washed with water, then heated in a microwave in 10mM sodium citrate pH 6.0 at 780W for 5 minutes, followed by 450W for 10 minutes. The sections were then incubated in methanol containing 30% hydrogen peroxide for 30 minutes and processed further like the cryosections. {Jussila, 1998}.

      Western Blot: antibody recognizes VEGFR-3 on reduced samples of HUVEC and HDMEC cells {primay human umbilical vein endothelial cells & primary human dermal microvascular endothelial cells}. The bands seen at molecular weight 195kDa and 125kDa represent the uncleaved and proteolytically cleaved forms of VEGFR3. The band at 175kDa is considered to be intracellular, unglycosylated precursor {Bando, 2004}.

      Immunoprecipitation: 1-5μL per 300-500μL of cell lysate. Use protein A or rabbit anti-mouse IgG capture for best results.

      ELISA: 9D9F9 has been used as the detection antibody for a VEGFR-3 ELISA {Bando, 2004}.

      Flow Cytometry: reactive on transfected NIH-3T3 cells expressing hVEGFR3, Other cell lines untested. {Jussila, 1998}.

      Optimal dilutions will need to be determined by the enduser.
      Biological Information
      ImmunogenRecombinantly produced, purified, hVEGFR-3 extracellular domain protein from baculovirus culture {Jussila, 1998}.
      Epitopeextracellular domain
      Clone9D9F9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecific for human VEGFR-3. Western blot and ELISA demonstrate that the antibody is highly specific for VEGFR-3 with no cross-reactivity with human VEGFR-1 or VEGFR-2 protein {Bando, 2004}. Cross reactivity with other species VEGFR-3 proteins untested.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VEGFR3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight175 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-VEGF RECEPTOR-3 MONOCLONAL ANTIBODY - 2390524 2390524
      MOUSE ANTI-VEGF RECEPTOR-3 - 2506455 2506455
      MOUSE ANTI-VEGF RECEPTOR-3 -2664467 2664467
      MOUSE ANTI-VEGF RECEPTOR-3 -2688642 2688642
      MOUSE ANTI-VEGF RECEPTOR-3 -2727279 2727279
      MOUSE ANTI-VEGF RECEPTOR-3 -2762325 2762325
      MOUSE ANTI-VEGF RECEPTOR-3 MONOCLONAL ANTIBODY - 2021729 2021729
      MOUSE ANTI-VEGF RECEPTOR-3 MONOCLONAL ANTIBODY - 2049233 2049233
      MOUSE ANTI-VEGF RECEPTOR-3 MONOCLONAL ANTIBODY - 2110497 2110497
      MOUSE ANTI-VEGF RECEPTOR-3 MONOCLONAL ANTIBODY - 2189712 2189712
      MOUSE ANTI-VEGF RECEPTOR-3 MONOCLONAL ANTIBODY - 2246768 2246768
      MOUSE ANTI-VEGF RECEPTOR-3 MONOCLONAL ANTIBODY - 2324571 2324571

      References

      Reference overviewApplicationPub Med ID
      Inhibition of FAK and VEGFR-3 binding decreases tumorigenicity in neuroblastoma.
      Stewart, JE; Ma, X; Megison, M; Nabers, H; Cance, WG; Kurenova, EV; Beierle, EA
      Molecular carcinogenesis 54 9-23 2015

      Show Abstract
      23868727 23868727
      Regorafenib (BAY 73-4506): antitumor and antimetastatic activities in preclinical models of colorectal cancer.
      Schmieder, R; Hoffmann, J; Becker, M; Bhargava, A; Müller, T; Kahmann, N; Ellinghaus, P; Adams, R; Rosenthal, A; Thierauch, KH; Scholz, A; Wilhelm, SM; Zopf, D
      International journal of cancer. Journal international du cancer 135 1487-96 2014

      Show Abstract
      24347491 24347491
      Inhibition of the focal adhesion kinase and vascular endothelial growth factor receptor-3 interaction leads to decreased survival in human neuroblastoma cell lines.
      Beierle, EA; Ma, X; Stewart, JE; Megison, M; Cance, WG; Kurenova, EV
      Molecular carcinogenesis 53 230-42 2014

      Show Abstract
      Western Blotting23065847 23065847
      VEGF-C, VEGF-D and VEGFR-3 expression in peripheral neuroblastic tumours.
      Ramani, Pramila, et al.
      Histopathology, 61: 1006-16 (2012) 2012

      Show Abstract
      22804730 22804730
      Different patterns of NF-κB and Notch1 signaling contribute to tumor-induced lymphangiogenesis of esophageal squamous cell carcinoma.
      Su, C; Chen, Z; Luo, H; Su, Y; Liu, W; Cai, L; Wang, T; Lei, Y; Zhong, B
      Journal of experimental & clinical cancer research : CR 30 85 2011

      Show Abstract Full Text Article
      21939555 21939555
      Vascular endothelial growth factor receptor-3 promotes breast cancer cell proliferation, motility and survival in vitro and tumor formation in vivo.
      Elena V Kurenova,Darrell L Hunt,Dihua He,Ann D Fu,Nicole A Massoll,Vita M Golubovskaya,Christopher A Garces,William G Cance
      Cell cycle (Georgetown, Tex.) 8 2009

      Show Abstract
      19556880 19556880
      VEGFR-3 and its ligand VEGF-C are associated with angiogenesis in breast cancer.
      Valtola, R, et al.
      Am. J. Pathol., 154: 1381-90 (1999) 1999

      Show Abstract
      10329591 10329591

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

      Data Sheet

      Title
      Anti-VEGF Receptor-3, extracellular domain, clone 9D9F9 - Data Sheet

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      Categories

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