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ABS82 | Anti-VEGF Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Pm WB, IH(P) Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABS82
      DescriptionAnti-VEGF Antibody
      Alternate Names
      • vascular endothelial growth factor A
      • vascular endothelial growth factor
      • Vascular permeability factor
      • vascular endothelial growth factor isoform VEGF
      Background InformationVEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
      Product Information
      FormatAffinity Purified
      • HuVec cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect VEGF using this Anti-VEGF Antibody validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: 1:400 dilution from a previous lot detected VEGF in normal human placental villi tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes VEGF at the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Primate
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with primate based on 100% sequence homology.
      Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq].
      Gene Symbol
      • VPF
      • VEGF-A
      • MVCD
      • VEGF
      • VEGA
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.

      SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).

      SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

      ALTERNATIVE PRODUCTS: 10 named isoforms [FASTA] produced by alternative splicing.
      Molecular Weight~ 55 kDa observed. UniProtKB/Swiss-Prot P15692 (VEGFA_HUMAN) entry describes 13 isoforms produced by alternative promoter usage, alternative splicing and alternative initiation: Isoform 1 at 27.042 kDa, Isoform 2 at 25.173 kDa, Isoform 3 at 24.422 kDa, Isoform 4 at 22.314 kDa, Isoform 5 at 20.218 kDa, Isoform 6 at 20.064 kDa, Isoform 7 at 22.259 kDa, Isoform 8 at 17.219 kDa, Isoform 9 at 15.981 kDa, Isoform 10 at 40.738 kDa, Isoform 11 at 35.643 kDa, Isoform 12 at 43.597 kDa, Isoform 13 at 45.467 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HuVec cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected VEGF on 10 µg of HuVec cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-VEGF - 2142671 2142671
      Anti-VEGF - 2370846 2370846
      Anti-VEGF - 2436810 2436810
      Anti-VEGF - 2452470 2452470
      Anti-VEGF - 2461591 2461591
      Anti-VEGF - 1956737 1956737
      Anti-VEGF - 2009447 2009447
      Anti-VEGF - 2055475 2055475
      Anti-VEGF - 2211971 2211971
      Anti-VEGF - 2275643 2275643
      Anti-VEGF - 2298402 2298402
      Anti-VEGF - JBC1787366 JBC1787366
      Anti-VEGF - NG1813629 NG1813629
      Anti-VEGF - NG1840153 NG1840153
      Anti-VEGF - NG1853807 NG1853807
      Anti-VEGF - NG1886558 NG1886558
      Anti-VEGF - NRG1765535 NRG1765535
      Anti-VEGF -2490684 2490684
      Anti-VEGF -2615009 2615009
      Anti-VEGF -2654301 2654301
      Anti-VEGF -2688591 2688591
      Anti-VEGF -2787650 2787650
      Anti-VEGF -2814784 2814784


      Reference overviewPub Med ID
      Akt/hypoxia-inducible factor-1α signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model.
      Jing, L; Li, S; Li, Q
      Experimental and therapeutic medicine 9 2141-2146 2015

      Show Abstract
      26136949 26136949
      Effects of prenatal hypoxia on schizophrenia-related phenotypes in heterozygous reeler mice: a gene × environment interaction study.
      Howell, KR; Pillai, A
      European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 24 1324-36 2014

      Show Abstract
      24946696 24946696
      Amount of torque and duration of stretching affects correction of knee contracture in a rat model of spinal cord injury.
      Moriyama, H; Tobimatsu, Y; Ozawa, J; Kito, N; Tanaka, R
      Clinical orthopaedics and related research 471 3626-36 2013

      Show Abstract
      23893364 23893364
      Airway administration of vascular endothelial growth factor siRNAs induces transient airspace enlargement in mice.
      Takahashi, Y; Izumi, Y; Kohno, M; Ikeda, E; Nomori, H
      International journal of medical sciences 10 1702-14 2013

      Show Abstract
      24155658 24155658
      Local injections of adipose-derived mesenchymal stem cells modulate inflammation and increase angiogenesis ameliorating the dystrophic phenotype in dystrophin-deficient skeletal muscle.
      Carlos Hermano da Justa Pinheiro,Jean César Farias de Queiroz,Lucas Guimarães-Ferreira,Kaio Fernando Vitzel,Renato Tadeu Nachbar,Luís Gustavo Oliveira de Sousa,Alcione Lescano de Souza-Jr,Maria Tereza Nunes,Rui Curi,Carlos Hermano da Justa Pinheiro,Jean C de Queiroz,Lucas Guimar,Lu de Sousa
      Stem cell reviews 8 2012

      Show Abstract
      21874281 21874281
      A bioreactor for subjecting cultured cells to fast-rate intermittent hypoxia.
      Theodora Tsapikouni,Elena Garreta,Esther Melo,Daniel Navajas,Ramon Farré
      Respiratory physiology & neurobiology 182 2012

      Show Abstract
      22246085 22246085


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