ST1553 Anti-VCP Mouse mAb (4A8)

ST1553
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1553-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
          Catalogue NumberST1553
          Brand Family Calbiochem®
          SynonymsAnti-Valosin-Containing Protein
          Application Data
          Detection of human VCP by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human VCP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (3 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2.
          Positive controlHePG2 cells, human placenta tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
          Clone4A8
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-VCP Mouse mAb (4A8) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-November-2010 RFH
          SynonymsAnti-Valosin-Containing Protein
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human VCP by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human VCP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (3 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
          BackgroundVCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
          HostMouse
          ImmunogenFull-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
          Clone4A8
          IsotypeIgG2a
          Specieshuman
          Positive controlHePG2 cells, human placenta tissue
          Formliquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review