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05-1312 | Anti-Ubiquityl-Histone H2B Antibody, clone 56

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, ChIP M Purified Monoclonal Antibody
      Catalogue Number05-1312
      Trade Name
      • Upstate
      DescriptionAnti-Ubiquityl-Histone H2B Antibody, clone 56
      Alternate Names
      • H2BUb
      • Histone H2B (ubiquityl)
      • HIST1H2B
      • H2BUbK119
      • H2BK119Ub
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression of several yeast genes.
      Product Information
      • HeLa cell lysate.
      PresentationPurified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
      ApplicationAnti-Ubiquityl-Histone H2B Antibody, clone 56 is a Mouse Monoclonal Antibody for detection of Ubiquityl-Histone H2B also known as H2BUb, Histone H2B (ubiquityl), HIST1H2B & has been validated in WB.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Biological Information
      ImmunogenImmunogen used was a branched peptide corresponding to the ubiquitination site on human H2B.
      EpitopeUbiquityl Lys120
      Cloneclone 56
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes histone H2B when it is ubiquitinated at Lys120 but not H2B in its unmodified state, or ubiquitin unconjugated to H2B. Mr ~25 kDa.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteBased on sequence homology, broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
      • Ubiquitylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

      Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      Subcellular location: Nucleus.

      Post-translational modification: Monoubiquitination of Lys-120 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
      Molecular Weight~25 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot

      Western Blot Analysis:
      A 0.25 μg/mL dilution of a recent lot detected ubiquityl histone H2B in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ubiquityl-Histone H2B, clone 56 - 2128268 2128268
      Anti-Ubiquityl-Histone H2B, clone 56 - 2387811 2387811
      Anti-Ubiquityl-Histone H2B, clone 56 - 2452487 2452487
      Anti-Ubiquityl-Histone H2B, clone 56 - NG1856943 NG1856943
      Anti-Ubiquityl-Histone H2B, clone 56 - 1964536 1964536
      Anti-Ubiquityl-Histone H2B, clone 56 - 2049280 2049280
      Anti-Ubiquityl-Histone H2B, clone 56 - 2194093 2194093
      Anti-Ubiquityl-Histone H2B, clone 56 - 2228143 2228143
      Anti-Ubiquityl-Histone H2B, clone 56 - 2309343 2309343
      Anti-Ubiquityl-Histone H2B, clone 56 - 2491202 2491202
      Anti-Ubiquityl-Histone H2B, clone 56 - DAM1567819 DAM1567819
      Anti-Ubiquityl-Histone H2B, clone 56 - DAM1588291 DAM1588291
      Anti-Ubiquityl-Histone H2B, clone 56 - JBC1771118 JBC1771118
      Anti-Ubiquityl-Histone H2B, clone 56 - NG1543098 NG1543098
      Anti-Ubiquityl-Histone H2B, clone 56 - NG1661335 NG1661335
      Anti-Ubiquityl-Histone H2B, clone 56 - NG1694277 NG1694277
      Anti-Ubiquityl-Histone H2B, clone 56 - NG1726934 NG1726934
      Anti-Ubiquityl-Histone H2B, clone 56 - NG1808605 NG1808605
      Anti-Ubiquityl-Histone H2B, clone 56 - NG1845586 NG1845586
      Anti-Ubiquityl-Histone H2B, clone 56 -2567064 2567064
      Anti-Ubiquityl-Histone H2B, clone 56 -2620090 2620090
      Anti-Ubiquityl-Histone H2B, clone 56 -2654212 2654212
      Anti-Ubiquityl-Histone H2B, clone 56 -2717674 2717674

      References | 19 Available | See All References

      Reference overviewApplicationPub Med ID
      The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
      Yang, W; Lee, YH; Jones, AE; Woolnough, JL; Zhou, D; Dai, Q; Wu, Q; Giles, KE; Townes, TM; Wang, H
      Nature communications 5 3818 2014

      Show Abstract
      24784029 24784029
      Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration.
      Mohan, RD; Dialynas, G; Weake, VM; Liu, J; Martin-Brown, S; Florens, L; Washburn, MP; Workman, JL; Abmayr, SM
      Genes & development 28 259-72 2014

      Show Abstract
      24493646 24493646
      Transcription factor Sp3 represses expression of p21CIP¹ via inhibition of productive elongation by RNA polymerase II.
      Valin, A; Ouyang, J; Gill, G
      Molecular and cellular biology 33 1582-93 2013

      Show Abstract
      23401853 23401853
      Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemia.
      Wang, E; Kawaoka, S; Yu, M; Shi, J; Ni, T; Yang, W; Zhu, J; Roeder, RG; Vakoc, CR
      Proceedings of the National Academy of Sciences of the United States of America 110 3901-6 2013

      Show Abstract
      23412334 23412334
      ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
      Wu, L; Lee, SY; Zhou, B; Nguyen, UT; Muir, TW; Tan, S; Dou, Y
      Molecular cell 49 1108-20 2013

      Show Abstract
      23453805 23453805
      The deubiquitylating enzyme USP44 counteracts the DNA double-strand break response mediated by the RNF8 and RNF168 ubiquitin ligases.
      Mosbech, A; Lukas, C; Bekker-Jensen, S; Mailand, N
      The Journal of biological chemistry 288 16579-87 2013

      Show Abstract
      23615962 23615962
      The ZFP-1(AF10)/DOT-1 complex opposes H2B ubiquitination to reduce Pol II transcription.
      Cecere, G; Hoersch, S; Jensen, MB; Dixit, S; Grishok, A
      Molecular cell 50 894-907 2013

      Show Abstract
      Western Blotting23806335 23806335
      USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing.
      Zhang, Z; Jones, A; Joo, HY; Zhou, D; Cao, Y; Chen, S; Erdjument-Bromage, H; Renfrow, M; He, H; Tempst, P; Townes, TM; Giles, KE; Ma, L; Wang, H
      Genes & development 27 1581-95 2013

      Show Abstract
      23824326 23824326
      Decreased histone H2B monoubiquitination in malignant gastric carcinoma.
      Wang, ZJ; Yang, JL; Wang, YP; Lou, JY; Chen, J; Liu, C; Guo, LD
      World journal of gastroenterology 19 8099-107 2013

      Show Abstract
      24307806 24307806
      The interaction of the von Hippel-Lindau tumor suppressor and heterochromatin protein 1.
      Lai, Y; Song, M; Hakala, K; Weintraub, ST; Shiio, Y
      Archives of biochemistry and biophysics 518 103-10 2012

      Show Abstract
      22234250 22234250


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