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MAB1510 | Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IH(P), IP, WB M Ascites Monoclonal Antibody
      Catalogue NumberMAB1510
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)
      Alternate Names
      • Ubiquitin
      • ubiquitin-CEP80
      Background InformationUbiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
      Product Information
      • Alzheimer's diseased brain.
      PresentationMouse monoclonal IgG1κ in buffer containing containing 10 mM sodium azide.
      ApplicationThis Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS) is validated for use in IH(P), IP, WB for the detection of Ubiquitin.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry (frozen or paraffin embedded sections):
      1:250-1:1,000 dilution from a previous lot was used.

      Electron Microscopy:
      1:250-1:1,000 dilution from a previous lot was used.

      (Liang, 1995; Oberdorf, 1999)
      Note: Suggested blocking agent is bovine serum albumin.

      1:250-1:1,000. The sensitivity of the assay can be enhanced by autoclaving the blot (Swerdlow, 1986).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified ubiquitin (bovine RBC) covalently coupled to KLH using glutaraldehyde.
      CloneUbi-1 (aka 042691GS)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityUbiquitin (76 amino acid, 8.5 kDa) both conjugated and unconjugated. Shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson’s disease) and Pick bodies (in Pick’s disease).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteExpected to cross- react with Bovine, Chicken, and Drosophila. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
      Gene Symbol
      • RPS27A
      • UBA80
      • UBC
      • CEP80
      • Uba80
      • UBCEP80
      • UBCEP1:UBA52
      • UBB
      • HUBCEP80
      • UBCEP2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
      SIZE: 76 amino acids; 8565 Da
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
      PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
      SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
      MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
      Molecular Weight8.5 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Jurkat lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected UBIQUITIN on 10 μg of Jurkat3 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquotes from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 2366524 2366524
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 2390415 2390415
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - NG1940102 NG1940102
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 1997908 1997908
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 2028275 2028275
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 2090916 2090916
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 2262649 2262649
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 2273806 2273806
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - 2475634 2475634
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - LV1473066 LV1473066
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - LV1581095 LV1581095
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - NG1657540 NG1657540
      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - NG1723960 NG1723960
      Anti-Ubiquitin, clone Ubi-1 -2524709 2524709
      Anti-Ubiquitin, clone Ubi-1 -2581774 2581774
      Anti-Ubiquitin, clone Ubi-1 -2594861 2594861
      Anti-Ubiquitin, clone Ubi-1 -2653141 2653141
      Anti-Ubiquitin, clone Ubi-1 -2681282 2681282
      Anti-Ubiquitin, clone Ubi-1 -2701610 2701610
      Anti-Ubiquitin, clone Ubi-1 -2713245 2713245
      Anti-Ubiquitin, clone Ubi-1 -2746558 2746558
      Anti-Ubiquitin, clone Ubi-1 -2795028 2795028
      Anti-Ubiquitin, clone Ubi-1 -NG2524709 NG2524709

      References | 99 Available | See All References

      Reference overviewApplicationPub Med ID
      Distinct partitioning of ALS associated TDP-43, FUS and SOD1 mutants into cellular inclusions.
      Farrawell, NE; Lambert-Smith, IA; Warraich, ST; Blair, IP; Saunders, DN; Hatters, DM; Yerbury, JJ
      Scientific reports 5 13416 2015

      Show Abstract
      26293199 26293199
      BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5.
      Qin, J; Zhou, Z; Chen, W; Wang, C; Zhang, H; Ge, G; Shao, M; You, D; Fan, Z; Xia, H; Liu, R; Chen, C
      Nature communications 6 8471 2015

      Show Abstract
      26419610 26419610
      Apelin promotes diabetic nephropathy by inducing podocyte dysfunction via inhibiting proteasome activities.
      Guo, C; Liu, Y; Zhao, W; Wei, S; Zhang, X; Wang, W; Zeng, X
      Journal of cellular and molecular medicine 19 2273-85 2015

      Show Abstract
      26103809 26103809
      Trehalose reverses cell malfunction in fibroblasts from normal and Huntington's disease patients caused by proteosome inhibition.
      Fernandez-Estevez, MA; Casarejos, MJ; López Sendon, J; Garcia Caldentey, J; Ruiz, C; Gomez, A; Perucho, J; de Yebenes, JG; Mena, MA
      PloS one 9 e90202 2014

      Show Abstract
      24587280 24587280
      A DERL3-associated defect in the degradation of SLC2A1 mediates the Warburg effect.
      Lopez-Serra, P; Marcilla, M; Villanueva, A; Ramos-Fernandez, A; Palau, A; Leal, L; Wahi, JE; Setien-Baranda, F; Szczesna, K; Moutinho, C; Martinez-Cardus, A; Heyn, H; Sandoval, J; Puertas, S; Vidal, A; Sanjuan, X; Martinez-Balibrea, E; Viñals, F; Perales, JC; Bramsem, JB; Ørntoft, TF; Andersen, CL; Tabernero, J; McDermott, U; Boxer, MB; Vander Heiden, MG; Albar, JP; Esteller, M
      Nature communications 5 3608 2014

      Show Abstract
      24699711 24699711
      PINK1 phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity.
      Kane, LA; Lazarou, M; Fogel, AI; Li, Y; Yamano, K; Sarraf, SA; Banerjee, S; Youle, RJ
      The Journal of cell biology 205 143-53 2014

      Show Abstract
      24751536 24751536
      Astrocytic TDP-43 pathology in Alexander disease.
      Walker, AK; Daniels, CM; Goldman, JE; Trojanowski, JQ; Lee, VM; Messing, A
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 6448-58 2014

      Show Abstract
      24806671 24806671
      Possible involvement of lysosomal dysfunction in pathological changes of the brain in aged progranulin-deficient mice.
      Tanaka, Y; Chambers, JK; Matsuwaki, T; Yamanouchi, K; Nishihara, M
      Acta neuropathologica communications 2 78 2014

      Show Abstract
      25022663 25022663
      MTOR-independent, autophagic enhancer trehalose prolongs motor neuron survival and ameliorates the autophagic flux defect in a mouse model of amyotrophic lateral sclerosis.
      Zhang, X; Chen, S; Song, L; Tang, Y; Shen, Y; Jia, L; Le, W
      Autophagy 10 588-602 2014

      Show Abstract
      24441414 24441414
      Optineurin immunoreactivity in neuronal and glial intranuclear inclusions in adult-onset neuronal intranuclear inclusion disease.
      Nakamura, M; Murray, ME; Lin, WL; Kusaka, H; Dickson, DW
      American journal of neurodegenerative disease 3 93-102 2014

      Show Abstract
      Immunofluorescence25232514 25232514

      Data Sheet

      Anti-Ubiquitin, clone Ubi-1 (aka 042691GS) - Data Sheet

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