DR1047 Anti-UHRF1 Mouse mAb (3A11)

DR1047
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DR1047-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.
          Catalogue NumberDR1047
          Brand Family Calbiochem®
          SynonymsAnti-Ubiquitin-Like PHD/RING Finger Domain 1
          Application Data
          Detection of human UHRF1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human UHRF1 by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human endometrium tissue. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human UHRF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (10 µg/ml). Detection: fluorescence.
          References
          ReferencesMistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2.
          Positive controlHeLa cells, Human endometrium tissue
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
          ImmunogenNA
          Clone3A11
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-UHRF1 Mouse mAb (3A11) SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Mistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-November-2010 RFH
          SynonymsAnti-Ubiquitin-Like PHD/RING Finger Domain 1
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human UHRF1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human UHRF1 by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human endometrium tissue. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human UHRF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (10 µg/ml). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.
          BackgroundThis protein is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIα and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint
          HostMouse
          Immunogen speciesNA
          ImmunogenA recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
          Clone3A11
          IsotypeIgG₁
          Specieshuman
          Positive controlHeLa cells, Human endometrium tissue
          Formliquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review
          ReferencesMistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.