MAB318 Anti-Tyrosine Hydroxylase Antibody, clone LNC1

MAB318
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, Fg, H, M, Mk, R, Lz, Vo, Zebrafish IHC, IH(P), IP, WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB318
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody, clone LNC1
      Alternate Names
      • TH
      • Tyrosine Monooxygenase
      Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatAscites
      Control
      • Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
      PresentationAscites mouse monoclonal IgG1κ fluid containing 3% BSA. Contains no preservative.
      Applications
      ApplicationDetect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry(paraffin):
      A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.

      Immunoprecipitation:
      A previous lot of this antibody was used in IP (Perez, 2002).

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenTyrosine Hydroxylase purified from PC12 cells
      CloneLNC1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).
      IsotypeIgG1κ
      Species Reactivity
      • Chicken
      • Frog
      • Human
      • Mouse
      • Monkey
      • Rat
      • Lizard
      • Vole
      • Zebrafish
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight59-63 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western blot:
      1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase, clone LNC12464515
      Anti-Tyrosine Hydroxylase, clone LNC1 - 21277812127781
      Anti-Tyrosine Hydroxylase, clone LNC1 - 23665312366531
      Anti-Tyrosine Hydroxylase, clone LNC1 - 24301292430129
      Anti-Tyrosine Hydroxylase, clone LNC1 - 20030012003001
      Anti-Tyrosine Hydroxylase, clone LNC1 - 20614302061430
      Anti-Tyrosine Hydroxylase, clone LNC1 - 22119272211927
      Anti-Tyrosine Hydroxylase, clone LNC1 - 22763082276308
      Anti-Tyrosine Hydroxylase, clone LNC1 - 24995572499557
      Anti-Tyrosine Hydroxylase, clone LNC1 - 29507382950738

      References

      Reference overviewApplicationSpeciesPub Med ID
      The temporal expression pattern of alpha-synuclein modulates olfactory neurogenesis in transgenic mice.
      Schreglmann, SR; Regensburger, M; Rockenstein, E; Masliah, E; Xiang, W; Winkler, J; Winner, B
      PloS one  10  e0126261  2015

      Show Abstract
      25961568 25961568
      Lack of additive role of ageing in nigrostriatal neurodegeneration triggered by α-synuclein overexpression.
      Bourdenx, M; Dovero, S; Engeln, M; Bido, S; Bastide, MF; Dutheil, N; Vollenweider, I; Baud, L; Piron, C; Grouthier, V; Boraud, T; Porras, G; Li, Q; Baekelandt, V; Scheller, D; Michel, A; Fernagut, PO; Georges, F; Courtine, G; Bezard, E; Dehay, B
      Acta neuropathologica communications  3  46  2015

      Show Abstract
      26205255 26205255
      Prefrontal dopamine regulates fear reinstatement through the downregulation of extinction circuits.
      Hitora-Imamura, N; Miura, Y; Teshirogi, C; Ikegaya, Y; Matsuki, N; Nomura, H
      eLife  4  2015

      Show Abstract
      26226637 26226637
      Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation.
      Jiang, YH; Jiang, P; Yang, JL; Ma, DF; Lin, HQ; Su, WG; Wang, Z; Li, X
      PloS one  10  e0133971  2015

      Show Abstract
      26230083 26230083
      Comprehensive functional characterization of murine infantile Batten disease including Parkinson-like behavior and dopaminergic markers.
      Dearborn, JT; Harmon, SK; Fowler, SC; O'Malley, KL; Taylor, GT; Sands, MS; Wozniak, DF
      Scientific reports  5  12752  2015

      Show Abstract
      26238334 26238334
      Viable neuronopathic Gaucher disease model in Medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein.
      Uemura, N; Koike, M; Ansai, S; Kinoshita, M; Ishikawa-Fujiwara, T; Matsui, H; Naruse, K; Sakamoto, N; Uchiyama, Y; Todo, T; Takeda, S; Yamakado, H; Takahashi, R
      PLoS genetics  11  e1005065  2015

      Show Abstract
      Immunohistochemistry25835295 25835295
      Inner retinal change in a novel rd1-FTL mouse model of retinal degeneration.
      Greferath, U; Anderson, EE; Jobling, AI; Vessey, KA; Martinez, G; de Iongh, RU; Kalloniatis, M; Fletcher, EL
      Frontiers in cellular neuroscience  9  293  2015

      Show Abstract
      26283925 26283925
      Localization of reelin signaling pathway components in murine midbrain and striatum.
      Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
      Cell and tissue research  359  393-407  2015

      Show Abstract
      Mouse25418135 25418135
      Catecholaminergic innervation of central and peripheral auditory circuitry varies with reproductive state in female midshipman fish, Porichthys notatus.
      Forlano, PM; Ghahramani, ZN; Monestime, CM; Kurochkin, P; Chernenko, A; Milkis, D
      PloS one  10  e0121914  2015

      Show Abstract
      25849450 25849450
      A distinct subtype of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment.
      Chand, AN; Galliano, E; Chesters, RA; Grubb, MS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  1573-90  2015

      Show Abstract
      25632134 25632134

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