MAB5280 Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15

MAB5280
40 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, Qu, R IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5280
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody, clone 2/40/15
      Alternate Names
      • TH
      • Tyrosine Monooxygenase
      Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue
      PresentationPurified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.

      Western blot:
      1:1000-1:2000

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).
      Clone2/40/15
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityTyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Chicken
      • Quail
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight56-60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size40 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase, clone -27102602710260
      Anti-Tyrosine Hydroxylase, clone -27898032789803
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 24304422430442
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 19750741975074
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 20069342006934
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 20439082043908
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 22049122204912
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 23187882318788
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 24911862491186
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - 25639922563992

      References

      Reference overviewApplicationSpeciesPub Med ID
      Patterns of Brain Activation and Meal Reduction Induced by Abdominal Surgery in Mice and Modulation by Rikkunshito.
      Wang, L; Mogami, S; Yakabi, S; Karasawa, H; Yamada, C; Yakabi, K; Hattori, T; Taché, Y
      PloS one  10  e0139325  2015

      Show Abstract
      26421719 26421719
      A role for nonapeptides and dopamine in nest-building behaviour.
      Hall, ZJ; Healy, SD; Meddle, SL
      Journal of neuroendocrinology  27  158-65  2015

      Show Abstract
      25514990 25514990
      Chronic nicotine activates stress/reward-related brain regions and facilitates the transition to compulsive alcohol drinking.
      Leão, RM; Cruz, FC; Vendruscolo, LF; de Guglielmo, G; Logrip, ML; Planeta, CS; Hope, BT; Koob, GF; George, O
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  6241-53  2015

      Show Abstract
      25878294 25878294
      Ultrastructural localization of tyrosine hydroxylase in tree shrew nucleus accumbens core and shell.
      McCollum, LA; Roberts, RC
      Neuroscience  271  23-34  2014

      Show Abstract
      24769226 24769226
      A population of glomerular glutamatergic neurons controls sensory information transfer in the mouse olfactory bulb.
      Tatti, R; Bhaukaurally, K; Gschwend, O; Seal, RP; Edwards, RH; Rodriguez, I; Carleton, A
      Nature communications  5  3791  2014

      Show Abstract
      24804702 24804702
      Development of attenuated baroreflexes in obese Zucker rats coincides with impaired activation of nucleus tractus solitarius.
      Guimaraes, PS; Huber, DA; Campagnole-Santos, MJ; Schreihofer, AM
      American journal of physiology. Regulatory, integrative and comparative physiology  306  R681-92  2014

      Show Abstract
      24573182 24573182
      Mapping of reinforcing and analgesic effects of the mu opioid agonist Endomorphin-1 in the ventral midbrain of the rat.
      Thomas C Jhou,Sheng-Ping Xu,Mary R Lee,Courtney L Gallen,Satoshi Ikemoto
      Psychopharmacology  224  2012

      Show Abstract
      22669129 22669129
      Stress stimulates production of catecholamines in rat adipocytes.
      R Kvetnansky,J Ukropec,M Laukova,B Manz,K Pacak,P Vargovic
      Cellular and molecular neurobiology  32  2012

      Show Abstract
      22402834 22402834
      Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease.
      Sánchez-Danés, A; Richaud-Patin, Y; Carballo-Carbajal, I; Jiménez-Delgado, S; Caig, C; Mora, S; Di Guglielmo, C; Ezquerra, M; Patel, B; Giralt, A; Canals, JM; Memo, M; Alberch, J; López-Barneo, J; Vila, M; Cuervo, AM; Tolosa, E; Consiglio, A; Raya, A
      EMBO molecular medicine  4  380-95  2012

      Show Abstract
      ImmunofluorescenceHuman22407749 22407749
      Dopamine pathology in schizophrenia: analysis of total and phosphorylated tyrosine hydroxylase in the substantia nigra.
      Perez-Costas, E; Melendez-Ferro, M; Rice, MW; Conley, RR; Roberts, RC
      Frontiers in psychiatry  3  31  2012

      Show Abstract
      22509170 22509170

      Data Sheet

      Title
      Anti-Tyrosine Hydroxylase, clone 2/40/15 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies