AB1542 Anti-Tyrosine Hydroxylase Antibody

AB1542
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ma, R, M IHC, WB Sh Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1542
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody
      Alternate Names
      • Tyrosine 3-hydroxylase
      • Tyrosine 3-hydroxylase
      • tyrosine hydroxylase
      Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
      PresentationPurified sheep polyclonal in buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
      Applications
      ApplicationAnti-Tyrosine Hydroxylase Antibody is an antibody against Tyrosine Hydroxylase for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry(peroxidase): A previous lot of this antibody was used at 1:1,000 dilution. The antibody gives specific labeling of noradrenergic axons in primate cerebral cortex (Brain Res., 1989, 500:313-324.).
      Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenNative tyrosine hydroxylase from rat pheochromocytoma (J. Biol. Chem.,1982, 257:9416-9423).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostSheep
      SpecificityTyrosine Hydroxylase (TH, Tyrosine Monooxygenase). Cross-reacts with all mammalian forms tested to date and some non-mammalian forms.
      Species Reactivity
      • Mammals
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].

      Transcript Variant: This variant (2) uses a different donor splice site at the first coding exon and is missing an adjacent in-frame coding exon compared to transcript variant 1, resulting in an isoform (b) missing a 31 aa segment compared to isoform a.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight~60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20 to -80°C in undiluted aliquots from date of receipt. Store in a NON-self-defrosting freezer.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase3076453
      Anti-Tyrosine Hydroxylase - 21159192115919
      Anti-Tyrosine Hydroxylase - 21369872136987
      Anti-Tyrosine Hydroxylase - 23864872386487
      Anti-Tyrosine Hydroxylase - 24303382430338
      Anti-Tyrosine Hydroxylase - 19706921970692
      Anti-Tyrosine Hydroxylase - 20318062031806
      Anti-Tyrosine Hydroxylase - 21884662188466
      Anti-Tyrosine Hydroxylase - 23245112324511
      Anti-Tyrosine Hydroxylase - 25192882519288

      References

      Reference overviewApplicationSpeciesPub Med ID
      Hypothalamic overexpression of mutant huntingtin causes dysregulation of brown adipose tissue.
      Soylu-Kucharz, R; Adlesic, N; Baldo, B; Kirik, D; Petersén, Å
      Scientific reports  5  14598  2015

      Show Abstract
      26419281 26419281
      Sexual dimorphism in the hypophysiotropic tyrosine hydroxylase-positive neurons in the preoptic area of the teleost, Clarias batrachus.
      Saha, S; Patil, S; Singh, U; Singh, O; Singru, PS
      Biology of sex differences  6  23  2015

      Show Abstract
      26557978 26557978
      Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.
      Zhang, S; Qi, J; Li, X; Wang, HL; Britt, JP; Hoffman, AF; Bonci, A; Lupica, CR; Morales, M
      Nature neuroscience  18  386-92  2015

      Show Abstract
      25664911 25664911
      Norepinephrine activates dopamine D4 receptors in the rat lateral habenula.
      Root, DH; Hoffman, AF; Good, CH; Zhang, S; Gigante, E; Lupica, CR; Morales, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  3460-9  2015

      Show Abstract
      25716845 25716845
      Coronin-1 and calcium signaling governs sympathetic final target innervation.
      Suo, D; Park, J; Young, S; Makita, T; Deppmann, CD
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  3893-902  2015

      Show Abstract
      25740518 25740518
      Genome-wide characterisation of Foxa1 binding sites reveals several mechanisms for regulating neuronal differentiation in midbrain dopamine cells.
      Metzakopian, E; Bouhali, K; Alvarez-Saavedra, M; Whitsett, JA; Picketts, DJ; Ang, SL
      Development (Cambridge, England)  142  1315-24  2015

      Show Abstract
      25804738 25804738
      A glutamatergic reward input from the dorsal raphe to ventral tegmental area dopamine neurons.
      Qi, J; Zhang, S; Wang, HL; Wang, H; de Jesus Aceves Buendia, J; Hoffman, AF; Lupica, CR; Seal, RP; Morales, M
      Nature communications  5  5390  2014

      Show Abstract
      25388237 25388237
      A conserved axon type hierarchy governing peripheral nerve assembly.
      Wang, L; Mongera, A; Bonanomi, D; Cyganek, L; Pfaff, SL; Nüsslein-Volhard, C; Marquardt, T
      Development (Cambridge, England)  141  1875-83  2014

      Show Abstract
      24700820 24700820
      2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is selectively toxic to primary dopaminergic neurons in vitro.
      Griggs, AM; Agim, ZS; Mishra, VR; Tambe, MA; Director-Myska, AE; Turteltaub, KW; McCabe, GP; Rochet, JC; Cannon, JR
      Toxicological sciences : an official journal of the Society of Toxicology  140  179-89  2014

      Show Abstract
      24718704 24718704
      Mesodiencephalic dopaminergic neuronal differentiation does not involve GLI2A-mediated SHH-signaling and is under the direct influence of canonical WNT signaling.
      Mesman, S; von Oerthel, L; Smidt, MP
      PloS one  9  e97926  2014

      Show Abstract
      24865218 24865218

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