AB152 Anti-Tyrosine Hydroxylase Antibody

AB152
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Fe, Ft, Sqd, Dr, Ml ELISA, IF, IHC, IH(P), IP, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB152
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody
      Alternate Names
      • Tyrosine 3-hydroxylase
      • Tyrosine 3-monooxygenase
      • tyrosine hydroxylase
      • TH
      Background InformationTyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatAffinity Purified
      Control
      • POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
        NEGATATIVE CONTROL: Liver.
      PresentationPurified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
      Applications
      ApplicationAnti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

      Immunofluorescence:
      A previous lot of this antibody was used at 1:100-1:1,000 dilution.

      Immunoprecipitation:
      A previous lot of this antibody was used on Immunoprecipitation.

      ELISA:
      A previous lot of this antibody was used in ELISA.

      Optimal dilutions must be determined by the end user.
      Biological Information
      ImmunogenDenatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
      HostRabbit
      SpecificityTyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Feline
      • Ferret
      • Squid
      • Drosophila
      • Mollusk
      Species Reactivity NoteRat, mouse, ferret, feline, Aplysia (marine mollusc). It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight62 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase3072361
      Anti-Tyrosine Hydroxylase - 21159182115918
      Anti-Tyrosine Hydroxylase - 23888292388829
      Anti-Tyrosine Hydroxylase - 23922252392225
      Anti-Tyrosine Hydroxylase - 24265182426518
      Anti-Tyrosine Hydroxylase - 24589912458991
      Anti-Tyrosine Hydroxylase - 19519191951919
      Anti-Tyrosine Hydroxylase - 19946511994651
      Anti-Tyrosine Hydroxylase - 20211942021194
      Anti-Tyrosine Hydroxylase - 20382572038257

      References

      Reference overviewApplicationSpeciesPub Med ID
      Localization of reelin signaling pathway components in murine midbrain and striatum.
      Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
      Cell and tissue research  359  393-407  2015

      Show Abstract
      Mouse25418135 25418135
      Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems.
      Hua, ZL; Emiliani, FE; Nathans, J
      Neural development  10  21  2015

      Show Abstract
      26395878 26395878
      The Progressive BSSG Rat Model of Parkinson's: Recapitulating Multiple Key Features of the Human Disease.
      Van Kampen, JM; Baranowski, DC; Robertson, HA; Shaw, CA; Kay, DG
      PloS one  10  e0139694  2015

      Show Abstract
      26439489 26439489
      Sexual dimorphism in the hypophysiotropic tyrosine hydroxylase-positive neurons in the preoptic area of the teleost, Clarias batrachus.
      Saha, S; Patil, S; Singh, U; Singh, O; Singru, PS
      Biology of sex differences  6  23  2015

      Show Abstract
      26557978 26557978
      Prenatal exposure to methylphenidate affects the dopamine system and the reactivity to natural reward in adulthood in rats.
      Lepelletier, FX; Tauber, C; Nicolas, C; Solinas, M; Castelnau, P; Belzung, C; Emond, P; Cortese, S; Faraone, SV; Chalon, S; Galineau, L
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Show Abstract
      25522388 25522388
      Biochemical, histopathological and morphological profiling of a rat model of early immune stimulation: relation to psychopathology.
      Kubesova, A; Tejkalova, H; Syslova, K; Kacer, P; Vondrousova, J; Tyls, F; Fujakova, M; Palenicek, T; Horacek, J
      PloS one  10  e0115439  2015

      Show Abstract
      Immunohistochemistry25602957 25602957
      Adolescent intermittent ethanol exposure is associated with increased risky choice and decreased dopaminergic and cholinergic neuron markers in adult rats.
      Boutros, N; Semenova, S; Liu, W; Crews, FT; Markou, A
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Show Abstract
      25612895 25612895
      A distinct subtype of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment.
      Chand, AN; Galliano, E; Chesters, RA; Grubb, MS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  1573-90  2015

      Show Abstract
      25632134 25632134
      Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.
      Zhang, S; Qi, J; Li, X; Wang, HL; Britt, JP; Hoffman, AF; Bonci, A; Lupica, CR; Morales, M
      Nature neuroscience  18  386-92  2015

      Show Abstract
      25664911 25664911
      A method for combining RNAscope in situ hybridization with immunohistochemistry in thick free-floating brain sections and primary neuronal cultures.
      Grabinski, TM; Kneynsberg, A; Manfredsson, FP; Kanaan, NM
      PloS one  10  e0120120  2015

      Show Abstract
      Immunohistochemistry25794171 25794171

      Brochure

      Title
      SNAP i.d. 2.0 System Brochure

      Technical Info

      Title
      Millipore Tools for Characterizing Induced Pluripotent Stem Cells

      Posters

      Title
      Dopamine Selectively Predisposes Dopaminergic Neurons In Primary Mesencephalic Cultures To Damage By An Oxidative Insult