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AB152 | Anti-Tyrosine Hydroxylase Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Fe, Ft, Sqd, Dr, Ml ELISA, IF, IHC, IH(P), IP, WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB152
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody
      Alternate Names
      • Tyrosine 3-hydroxylase
      • Tyrosine 3-monooxygenase
      • tyrosine hydroxylase
      • TH
      Background InformationTyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      Product Information
      FormatAffinity Purified
      • POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
      PresentationPurified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
      ApplicationAnti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

      A previous lot of this antibody was used at 1:100-1:1,000 dilution.

      A previous lot of this antibody was used on Immunoprecipitation.

      A previous lot of this antibody was used in ELISA.

      Optimal dilutions must be determined by the end user.
      Biological Information
      ImmunogenDenatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
      SpecificityTyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Feline
      • Ferret
      • Squid
      • Drosophila
      • Mollusk
      Species Reactivity NoteRat, mouse, ferret, feline, Aplysia (marine mollusc). It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight62 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase - 2115918 2115918
      Anti-Tyrosine Hydroxylase - 2388829 2388829
      Anti-Tyrosine Hydroxylase - 2392225 2392225
      Anti-Tyrosine Hydroxylase - 2426518 2426518
      Anti-Tyrosine Hydroxylase - 2458991 2458991
      Anti-Tyrosine Hydroxylase - 1951919 1951919
      Anti-Tyrosine Hydroxylase - 1994651 1994651
      Anti-Tyrosine Hydroxylase - 2021194 2021194
      Anti-Tyrosine Hydroxylase - 2038257 2038257
      Anti-Tyrosine Hydroxylase - 2043879 2043879
      Anti-Tyrosine Hydroxylase - 2066692 2066692
      Anti-Tyrosine Hydroxylase - 2159637 2159637
      Anti-Tyrosine Hydroxylase - 2219275 2219275
      Anti-Tyrosine Hydroxylase - 2278475 2278475
      Anti-Tyrosine Hydroxylase - 2287151 2287151
      Anti-Tyrosine Hydroxylase - 2322569 2322569
      Anti-Tyrosine Hydroxylase - 2328305 2328305
      Anti-Tyrosine Hydroxylase - JC1634159 JC1634159
      Anti-Tyrosine Hydroxylase - JC1658244 JC1658244
      Anti-Tyrosine Hydroxylase - JC1682878 JC1682878
      Anti-Tyrosine Hydroxylase - JC1693653 JC1693653
      Anti-Tyrosine Hydroxylase - LV1458671 LV1458671
      Anti-Tyrosine Hydroxylase - LV1591385 LV1591385
      Anti-Tyrosine Hydroxylase - NG1723896 NG1723896
      Anti-Tyrosine Hydroxylase - NG1752018 NG1752018
      Anti-Tyrosine Hydroxylase - NG1780584 NG1780584
      Anti-Tyrosine Hydroxylase - NG1830749 NG1830749
      Anti-Tyrosine Hydroxylase - NG1861503 NG1861503
      Anti-Tyrosine Hydroxylase - NG1899903 NG1899903
      Anti-Tyrosine Hydroxylase -2493925 2493925
      Anti-Tyrosine Hydroxylase -2552365 2552365
      Anti-Tyrosine Hydroxylase -2593900 2593900
      Anti-Tyrosine Hydroxylase -2639432 2639432
      Anti-Tyrosine Hydroxylase -2664972 2664972
      Anti-Tyrosine Hydroxylase -2665965 2665965
      Anti-Tyrosine Hydroxylase -2665966 2665966
      Anti-Tyrosine Hydroxylase -2695213 2695213
      Anti-Tyrosine Hydroxylase -2722866 2722866
      Anti-Tyrosine Hydroxylase -2739435 2739435
      Anti-Tyrosine Hydroxylase -2745367 2745367
      Anti-Tyrosine Hydroxylase -2776882 2776882
      Anti-Tyrosine Hydroxylase -2795024 2795024

      References | 341 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
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      Cell and tissue research 359 393-407 2015

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      Mouse25418135 25418135
      Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems.
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      PloS one 10 e0139694 2015

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      26439489 26439489
      Sexual dimorphism in the hypophysiotropic tyrosine hydroxylase-positive neurons in the preoptic area of the teleost, Clarias batrachus.
      Saha, S; Patil, S; Singh, U; Singh, O; Singru, PS
      Biology of sex differences 6 23 2015

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      26557978 26557978
      Prenatal exposure to methylphenidate affects the dopamine system and the reactivity to natural reward in adulthood in rats.
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      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP) 18 2015

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      Biochemical, histopathological and morphological profiling of a rat model of early immune stimulation: relation to psychopathology.
      Kubesova, A; Tejkalova, H; Syslova, K; Kacer, P; Vondrousova, J; Tyls, F; Fujakova, M; Palenicek, T; Horacek, J
      PloS one 10 e0115439 2015

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      Immunohistochemistry25602957 25602957
      Adolescent intermittent ethanol exposure is associated with increased risky choice and decreased dopaminergic and cholinergic neuron markers in adult rats.
      Boutros, N; Semenova, S; Liu, W; Crews, FT; Markou, A
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP) 18 2015

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      25612895 25612895
      A distinct subtype of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment.
      Chand, AN; Galliano, E; Chesters, RA; Grubb, MS
      The Journal of neuroscience : the official journal of the Society for Neuroscience 35 1573-90 2015

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      25632134 25632134
      Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.
      Zhang, S; Qi, J; Li, X; Wang, HL; Britt, JP; Hoffman, AF; Bonci, A; Lupica, CR; Morales, M
      Nature neuroscience 18 386-92 2015

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      25664911 25664911
      A method for combining RNAscope in situ hybridization with immunohistochemistry in thick free-floating brain sections and primary neuronal cultures.
      Grabinski, TM; Kneynsberg, A; Manfredsson, FP; Kanaan, NM
      PloS one 10 e0120120 2015

      Show Abstract
      Immunohistochemistry25794171 25794171


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