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MABN1091 | Anti-Tug Antibody, clone EPR8615, rabbit monoclonal

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC Rb Unpurified Monoclonal Antibody
      Catalogue NumberMABN1091
      DescriptionAnti-Tug Antibody, clone EPR8615, rabbit monoclonal
      Alternate Names
      • Tether containing UBX domain for GLUT4
      • Alveolar soft part sarcoma chromosomal region candidate gene 1 protein
      • Alveolar soft part sarcoma locus
      • Renal papillary cell carcinoma protein 17
      • UBX domain-containing protein 9
      Background InformationIn eukaryotic cells, protein secretion and the transport of materials between membrane-bounded organelles requires efficient and accurate delivery of carrier vesicles to their correct destination. Tug is a tethering protein. That means it secures its target protein in one location away from a membrane and then facilitates its release and redistribution in the membrane under another set of conditions. Tug is the short name for Tether containing UBX domain for GLUT4, TUG-UBL1, or also called in earlier literature, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17 and UBX domain-containing protein 9. Tug is encoded by the ASPSCR1 gene, also known as ASPL, RCC17, TUG, UBXD9, and UBXN9. Tug’s target is the glucose transporter GLUT4 and essential transporter in muscle and brain. Tug controls GLUT4’s position from intracellular surface and its release to the outer cellular surface. Tug binds directly and specifically to GLUT4 and retains it inside cells in the absence of insulin. In the presence of insulin, Tug dissociates from GLUT4 allowing GLUT4 and the vesicles containing it to move to outer surface and fulfill its transport function of bringing glucose into cells. Disruption of Tug expression or action either via knockdown or competition disrupts the normal GLUT4 disposition even in the absence of insulin and interestingly when function is disrupted GLUT4 degradation is accelerated via lysosomoal shuttling. EMD-Millipore’s Anti-Tug rabbit monoclonal antibody has been tested in western blot on human K562, 293T and SW480 cell lysates and in immunohistochemistry on paraffin embedded human colon tissue.
      Product Information
      • MCF-7, THP-1, HeLa, and HepG2 cell lysates
      PresentationRabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Tug using this rabbit monoclonal antibody, Anti-Tug Antibody, clone EPR8615 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected Tug in human colon tissue.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to human Tug.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ASPL
      • RCC17
      • TUG
      • UBXD9
      • UBXN9
      • ASPSCR1
      • Tug
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~75 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7, THP-1, HeLa, and HepG2 cell lysates.

      Western Blotting Analysis: A 1:10,000-50,000 dilution of this antibody detected MCF-7, THP-1, HeLa, and HepG2 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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