MAB1637 Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

MAB1637
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, B, H, M, Mk, Po, R, Sh ELISA, ICC, IHC, IH(P), IP, WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB1637
      Replaces04-1049
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)
      Alternate Names
      • Tubulin beta-III
      • tubulin, beta 3
      • tubulin, beta 4
      Background InformationTubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.
      References
      Product Information
      FormatAscites
      Control
      • Positive control: Brain tissue or neurons of any type.
        Negative control: Non neural tissue or Astrocytes.
      PresentationMouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
      Applications
      ApplicationAnti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB with more than 55 product citations.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

      Immunoprecipitation:
      A previous lot of this antibody was used on Immunoprecipitation.

      Immunocytochemistry:
      1:100 to 1:500 dilution from a previous lot was used on fixed cells.

      Immunoblotting:
      1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

      ELISA:
      A previous lot of this antibody was used on ELISA.

      Optimal working dilution must be determined by the end user.
      Biological Information
      ImmunogenA synthetic peptide corresponding to amino acids 443-450 (ESEAQGPK) of human class III beta-tubulin conjugated to KLH.
      EpitopeC-terminus
      CloneTU-20 (Similar to TUJ1)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Bovine
      • Human
      • Mouse
      • Monkey
      • Pig
      • Rat
      • Sheep
      Species Reactivity NoteMouse. Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • beta-4
      • MC1R
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 23922412392241
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 24203602420360
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 20204822020482
      Anti-Tubulin, beta III isoform, -25499742549974
      Anti-Tubulin, beta III isoform, -25702242570224
      Anti-Tubulin, beta III isoform, -25834062583406
      Anti-Tubulin, beta III isoform, -26048182604818
      Anti-Tubulin, beta III isoform, -26367212636721
      Anti-Tubulin, beta III isoform, -27010632701063
      Anti-Tubulin, beta III isoform, -27367372736737

      References

      Reference overviewApplicationSpeciesPub Med ID
      In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts.
      Choi, HW; Kim, JS; Hong, YJ; Song, H; Seo, HG; Do, JT
      Scientific reports  5  13559  2015

      Show Abstract
      26315499 26315499
      PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia.
      Radford, H; Moreno, JA; Verity, N; Halliday, M; Mallucci, GR
      Acta neuropathologica  130  633-42  2015

      Show Abstract
      26450683 26450683
      A novel method to derive and expand mice neural stem cells efficiently without neuro-sphere formation.
      Ma, ZZ; Fan, L; Huang, JL; Pan, XJ
      International journal of clinical and experimental medicine  8  12834-41  2015

      Show Abstract
      26550198 26550198
      Loss of non-coding RNA expression from the DLK1-DIO3 imprinted locus correlates with reduced neural differentiation potential in human embryonic stem cell lines.
      Mo, CF; Wu, FC; Tai, KY; Chang, WC; Chang, KW; Kuo, HC; Ho, HN; Chen, HF; Lin, SP
      Stem cell research & therapy  6  1  2015

      Show Abstract
      25559585 25559585
      Macromolecular organization and fine structure of the human basilar membrane - RELEVANCE for cochlear implantation.
      Liu, W; Atturo, F; Aldaya, R; Santi, P; Cureoglu, S; Obwegeser, S; Glueckert, R; Pfaller, K; Schrott-Fischer, A; Rask-Andersen, H
      Cell and tissue research  360  245-62  2015

      Show Abstract
      25663274 25663274
      Astroblastoma: beside being a tumor entity, an occasional phenotype of astrocytic gliomas?
      Mellai, M; Piazzi, A; Casalone, C; Grifoni, S; Melcarne, A; Annovazzi, L; Cassoni, P; Denysenko, T; Valentini, MC; Cistaro, A; Schiffer, D
      OncoTargets and therapy  8  451-60  2015

      Show Abstract
      Immunofluorescence25737639 25737639
      Genetic manipulation of reptilian embryos: toward an understanding of cortical development and evolution.
      Nomura, T; Yamashita, W; Gotoh, H; Ono, K
      Frontiers in neuroscience  9  45  2015

      Show Abstract
      25759636 25759636
      Genome-wide characterisation of Foxa1 binding sites reveals several mechanisms for regulating neuronal differentiation in midbrain dopamine cells.
      Metzakopian, E; Bouhali, K; Alvarez-Saavedra, M; Whitsett, JA; Picketts, DJ; Ang, SL
      Development (Cambridge, England)  142  1315-24  2015

      Show Abstract
      25804738 25804738
      Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology.
      Sun, Y; Florer, J; Mayhew, CN; Jia, Z; Zhao, Z; Xu, K; Ran, H; Liou, B; Zhang, W; Setchell, KD; Gu, J; Grabowski, GA
      PloS one  10  e0118771  2015

      Show Abstract
      Immunofluorescence25822147 25822147
      Glycaemic regulation and insulin secretion are abnormal in cystic fibrosis pigs despite sparing of islet cell mass.
      Uc, A; Olivier, AK; Griffin, MA; Meyerholz, DK; Yao, J; Abu-El-Haija, M; Buchanan, KM; Vanegas Calderón, OG; Abu-El-Haija, M; Pezzulo, AA; Reznikov, LR; Hoegger, MJ; Rector, MV; Ostedgaard, LS; Taft, PJ; Gansemer, ND; Ludwig, PS; Hornick, EE; Stoltz, DA; Ode, KL; Welsh, MJ; Engelhardt, JF; Norris, AW
      Clinical science (London, England : 1979)  128  131-42  2015

      Show Abstract
      25142104 25142104

      Brochure

      Title
      AXIS: Axon Investigation System

      Data Sheet

      Title
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - Data Sheet

      Posters

      Title
      Investigation of Axon Growth and Development Using Two Novel Assay Formats

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      Categories

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