AB3201 Anti-Tubulin Antibody, Detyrosinated

AB3201
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ma, Pl, Su ICC, IH(P), WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB3201
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tubulin Antibody, Detyrosinated
      Alternate Names
      • Glu Tubulin
      Background InformationMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Hela Whole Cell Lysate.
      PresentationPurified rabbit polyclonal in buffer containing liquid PBS.
      Applications
      ApplicationAnti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) and WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPeptide corresponding to the C-terminal of Glu tubulin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Mammals
      • Green Plants
      • Sea Urchin
      Species Reactivity NoteHuman, mouse, and rat. Expected to cross-react with mammals, sea urchin, plants, not yeast (Paturle-Lafanechere, L., 1991).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • MC1R
      • beta-4
      Non-Reactive Species
      • Yeast (S. cerevisiae)
      Purification MethodAffinity purified immunoglobulin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN: SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on L6 lysate lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tubulin, Detyrosinated3077390
      Anti-Tubulin, Detyrosinated3083049
      Anti-Tubulin, Detyrosinated2390350
      Anti-Tubulin, Detyrosinated3072359
      Anti-Tubulin, Detyrosinated - 20740222074022
      Anti-Tubulin, Detyrosinated - 23839532383953
      Anti-Tubulin, Detyrosinated - 24422132442213
      Anti-Tubulin, Detyrosinated - 19519171951917
      Anti-Tubulin, Detyrosinated - 19722741972274
      Anti-Tubulin, Detyrosinated - 19912301991230

      References

      Reference overviewApplicationSpeciesPub Med ID
      AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.
      Herms, A; Bosch, M; Reddy, BJ; Schieber, NL; Fajardo, A; Rupérez, C; Fernández-Vidal, A; Ferguson, C; Rentero, C; Tebar, F; Enrich, C; Parton, RG; Gross, SP; Pol, A
      Nature communications  6  7176  2015

      Show Abstract
      Immunofluorescence26013497 26013497
      Using the avian mutant talpid2 as a disease model for understanding the oral-facial phenotypes of oral-facial-digital syndrome.
      Schock, EN; Chang, CF; Struve, JN; Chang, YT; Chang, J; Delany, ME; Brugmann, SA
      Disease models & mechanisms  8  855-66  2015

      Show Abstract
      Immunoblotting (Western)26044959 26044959
      Shh Signaling through the Primary Cilium Modulates Rat Oligodendrocyte Differentiation.
      Falcón-Urrutia, P; Carrasco, CM; Lois, P; Palma, V; Roth, AD
      PloS one  10  e0133567  2015

      Show Abstract
      26218245 26218245
      A Mec17-Myosin II Effector Axis Coordinates Microtubule Acetylation and Actin Dynamics to Control Primary Cilium Biogenesis.
      Rao, Y; Hao, R; Wang, B; Yao, TP
      PloS one  9  e114087  2014

      Show Abstract
      25494100 25494100
      Effects of α-tubulin K40 acetylation and detyrosination on kinesin-1 motility in a purified system.
      Kaul, N; Soppina, V; Verhey, KJ
      Biophysical journal  106  2636-43  2014

      Show Abstract
      24940781 24940781
      The cellular and molecular etiology of the craniofacial defects in the avian ciliopathic mutant talpid2.
      Chang, CF; Schock, EN; O'Hare, EA; Dodgson, J; Cheng, HH; Muir, WM; Edelmann, RE; Delany, ME; Brugmann, SA
      Development (Cambridge, England)  141  3003-12  2014

      Show Abstract
      Immunohistochemistry25053433 25053433
      ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis.
      Goggolidou, P; Stevens, JL; Agueci, F; Keynton, J; Wheway, G; Grimes, DT; Patel, SH; Hilton, H; Morthorst, SK; DiPaolo, A; Williams, DJ; Sanderson, J; Khoronenkova, SV; Powles-Glover, N; Ermakov, A; Esapa, CT; Romero, R; Dianov, GL; Briscoe, J; Johnson, CA; Pedersen, LB; Norris, DP
      Development (Cambridge, England)  141  3966-77  2014

      Show Abstract
      25294941 25294941
      HIV-1 induces the formation of stable microtubules to enhance early infection.
      Sabo, Y; Walsh, D; Barry, DS; Tinaztepe, S; de Los Santos, K; Goff, SP; Gundersen, GG; Naghavi, MH
      Cell host & microbe  14  535-46  2013

      Show Abstract
      24237699 24237699
      α2-Chimaerin regulates a key axon guidance transition during development of the oculomotor projection.
      Clark, C; Austen, O; Poparic, I; Guthrie, S
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  16540-51  2013

      Show Abstract
      24133258 24133258
      Tubulin acetyltransferase αTAT1 destabilizes microtubules independently of its acetylation activity.
      Kalebic, N; Martinez, C; Perlas, E; Hublitz, P; Bilbao-Cortes, D; Fiedorczuk, K; Andolfo, A; Heppenstall, PA
      Molecular and cellular biology  33  1114-23  2013

      Show Abstract
      23275437 23275437

      Data Sheet

      Title
      Anti-Tubulin, Detyrosinated - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies