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AB1541 | Anti-Tryptophan Hydroxylase Antibody

100 µL  
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      Replacement Information
      Replacement InformationAB1541 is a recommended replacement for AB1556

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R IHC, WB Sh Affinity Purified Polyclonal Antibody
      Catalogue NumberAB1541
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tryptophan Hydroxylase Antibody
      Alternate Names
      • Tryptophan 5-monooxygenase 1
      • indoleacetic acid-5-hydroxylase
      • tryptophan 5-hydroxylase
      • tryptophan 5-monooxygenase
      • tryptophan hydroxylase (tryptophan 5-monooxygenase)
      • tryptophan hydroxylase 1
      Background InformationTryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin.
      Product Information
      FormatAffinity Purified
      • Brain tissue
      • PC12 lysates
      PresentationPurified sheep polyclonal liquid in 150 mM NaCl, 10 mM HEPES (pH 7.5), 100 μg/mL BSA and 50% glycerol.
      ApplicationDetect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry(peroxidase):
      Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteReacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the gut, pineal gland, spleen, and thymus.
      Gene Symbol
      • TPH1
      • TPRH
      • MGC119994
      • TRPH
      • TPH
      • EC
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryCOFACTOR:Fe(2+) ion.
      SIZE:444 amino acids; 50985 Da
      SUBUNIT:Multimer of identical subunits.
      TISSUE SPECIFICITY:Isoform 2 seems to be less widely expressed than isoform 1.
      SIMILARITY:SwissProt: P17752 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. & Contains 1 ACT domain.
      Molecular Weight55 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tryptophan Hydroxylase - 2142152 2142152
      Anti-Tryptophan Hydroxylase - 2387423 2387423
      Anti-Tryptophan Hydroxylase - 2453653 2453653
      Anti-Tryptophan Hydroxylase - NG1848352 NG1848352
      Anti-Tryptophan Hydroxylase - 1956379 1956379
      Anti-Tryptophan Hydroxylase - 1972784 1972784
      Anti-Tryptophan Hydroxylase - 1994652 1994652
      Anti-Tryptophan Hydroxylase - 2018056 2018056
      Anti-Tryptophan Hydroxylase - 2039760 2039760
      Anti-Tryptophan Hydroxylase - 2065979 2065979
      Anti-Tryptophan Hydroxylase - 2302140 2302140
      Anti-Tryptophan Hydroxylase - 2492095 2492095
      Anti-Tryptophan Hydroxylase - JBC1766717 JBC1766717
      Anti-Tryptophan Hydroxylase - JBC1780591 JBC1780591
      Anti-Tryptophan Hydroxylase - JC1610649 JC1610649
      Anti-Tryptophan Hydroxylase - JC1641497 JC1641497
      Anti-Tryptophan Hydroxylase - JC1668326 JC1668326
      Anti-Tryptophan Hydroxylase - LV1392889 LV1392889
      Anti-Tryptophan Hydroxylase - NG1721743 NG1721743
      Anti-Tryptophan Hydroxylase - NG1915696 NG1915696
      Anti-Tryptophan Hydroxylase -2506313 2506313
      Anti-Tryptophan Hydroxylase -2736689 2736689
      Anti-Tryptophan Hydroxylase -2809957 2809957
      ChIPAb+ CREB - NG1886542 NG1886542

      References | 25 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Effects of chronic fluoxetine treatment on neurogenesis and tryptophan hydroxylase expression in adolescent and adult rats.
      Klomp, A; Václavů, L; Meerhoff, GF; Reneman, L; Lucassen, PJ
      PloS one 9 e97603 2014

      Show Abstract
      Immunohistochemistry24827731 24827731
      Dorsal raphe neuroinflammation promotes dramatic behavioral stress dysregulation.
      Howerton, AR; Roland, AV; Bale, TL
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 7113-23 2014

      Show Abstract
      24849347 24849347
      Extrasynaptic glycine receptors of rodent dorsal raphe serotonergic neurons: a sensitive target for ethanol.
      Maguire, EP; Mitchell, EA; Greig, SJ; Corteen, N; Balfour, DJ; Swinny, JD; Lambert, JJ; Belelli, D
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 39 1232-44 2014

      Show Abstract
      24264816 24264816
      Central circuits regulating the sympathetic outflow to lumbar muscles in spinally transected mice by retrograde transsynaptic transport.
      Xiang, HB; Liu, C; Liu, TT; Xiong, J
      International journal of clinical and experimental pathology 7 2987-97 2014

      Show Abstract
      25031717 25031717
      Raphe GABAergic neurons mediate the acquisition of avoidance after social defeat.
      Challis, C; Boulden, J; Veerakumar, A; Espallergues, J; Vassoler, FM; Pierce, RC; Beck, SG; Berton, O
      The Journal of neuroscience : the official journal of the Society for Neuroscience 33 13978-88, 13988a 2013

      Show Abstract
      23986235 23986235
      Identification of unique release kinetics of serotonin from guinea-pig and human enterochromaffin cells.
      Raghupathi, R; Duffield, MD; Zelkas, L; Meedeniya, A; Brookes, SJ; Sia, TC; Wattchow, DA; Spencer, NJ; Keating, DJ
      The Journal of physiology 591 5959-75 2013

      Show Abstract
      24099799 24099799
      Activation patterns of cells in selected brain stem nuclei of more and less stress responsive rats in two animal models of PTSD - predator exposure and submersion stress.
      Robert Adamec,Mate Toth,Jozsef Haller,Jozsef Halasz,Jacqueline Blundell
      Neuropharmacology 62 2012

      Show Abstract
      21112345 21112345
      Reduction of serotonergic neurons in the dorsal raphe due to chronic prenatal administration of a tryptophan-free diet.
      Guadalupe M Flores-Cruz,Alfonso Escobar
      International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 30 2012

      Show Abstract
      22244887 22244887
      Effect of fluoxetine on the expression of tryptophan hydroxylase and 14-3-3 protein in the dorsal raphe nucleus and hippocampus of rat.
      Mi Ran Choi,Sejin Hwang,Geu Meum Park,Kyung Hwa Jung,Seok Hyeon Kim,Nando Dulal Das,Young Gyu Chai
      Journal of chemical neuroanatomy 43 2012

      Show Abstract
      22285725 22285725
      HDAC6 regulates glucocorticoid receptor signaling in serotonin pathways with critical impact on stress resilience.
      Espallergues, J; Teegarden, SL; Veerakumar, A; Boulden, J; Challis, C; Jochems, J; Chan, M; Petersen, T; Deneris, E; Matthias, P; Hahn, CG; Lucki, I; Beck, SG; Berton, O
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 4400-16 2012

      Show Abstract
      22457490 22457490

      Data Sheet

      Anti-Tryptophan Hydroxylase - Data Sheet

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