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AB5441 | Anti-Tropomyosin Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R WB, ICC, IHC Sh Affinity Purified Polyclonal Antibody
      Catalogue NumberAB5441
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tropomyosin Antibody
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in PBS.
      ApplicationThis Anti-Tropomyosin Antibody is validated for use in WB, IC, IH for the detection of Tropomyosin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern Blot: 1:200-1:1,000. The antibody reacts with the ~29 kDa Tropomyosin protein on mouse brain cytosol. An additional band at ~52-55 kDa may be seen depending on sample and antibody concentration used. It is thought that this band may be a dimer.
      Immunocytochemistry: 1:100-1:500
      Immunohistochemistry: 1:100-1:500

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the 9d exon from the alpha gene of tropomyosin.
      SpecificityRecognizes Tropomyosin 1, 2, 3, 5a, 5b, 6.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
      Gene Symbol
      • TPM1
      • CMH3
      • Tropomyosin-1
      • HTM-alpha
      • Alpha-tropomyosin
      • TMSA
      • C15orf13
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09493 # Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
      SIZE: 284 amino acids; 32709 Da
      SUBUNIT: Heterodimer of an alpha and a beta chain.
      TISSUE SPECIFICITY: Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients.
      DOMAIN: SwissProt: P09493 The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven- residues periodicity.
      DISEASE: SwissProt: P09493 # Defects in TPM1 are the cause of familial hypertrophic cardiomyopathy type 3 (CMH3) [MIM:115196]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy [MIM:192600] is a heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The prevalence of the disease in the general population is 0.2%. FHC is clinically heterogeneous, with inter- and intrafamilial variations ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      SIMILARITY: Belongs to the tropomyosin family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      SHEEP ANTI-TROPOMYOSIN 1, 2, 3, 5a, 5b, 6 POLYCLONAL ANTIBODY - 2118938 2118938
      SHEEP ANTI-TROPOMYOSIN 1, 2, 3, 5a, 5b, 6 POLYCLONAL ANTIBODY - 2141811 2141811
      SHEEP ANTI-TROPOMYOSIN 1, 2, 3, 5a, 5b, 6 - 2517346 2517346
      SHEEP ANTI-TROPOMYOSIN 1, 2, 3, 5a, 5b, 6 - 2565829 2565829
      SHEEP ANTI-TROPOMYOSIN 1, 2, 3, 5a, 5b, 6 -2673089 2673089
      SHEEP ANTI-TROPOMYOSIN 1, 2, 3, 5a, 5b, 6 -2787704 2787704


      Reference overviewPub Med ID
      Functional effects of mutations in the tropomyosin-binding sites of tropomodulin1 and tropomodulin3.
      Lewis, RA; Yamashiro, S; Gokhin, DS; Fowler, VM
      Cytoskeleton (Hoboken, N.J.) 71 395-411 2014

      Show Abstract
      24922351 24922351
      Injection of vessel-derived stem cells prevents dilated cardiomyopathy and promotes angiogenesis and endogenous cardiac stem cell proliferation in mdx/utrn-/- but not aged mdx mouse models for duchenne muscular dystrophy.
      Chun, JL; O'Brien, R; Song, MH; Wondrasch, BF; Berry, SE
      Stem cells translational medicine 2 68-80 2013

      Show Abstract
      23283493 23283493
      Tropomyosin assembly intermediates in the control of microfilament system turnover.
      Staffan Grenklo, Louise Hillberg, Li-Sophie Zhao Rathje, George Pinaev, Clarence E Schutt, Uno Lindberg, Staffan Grenklo, Louise Hillberg, Li-Sophie Zhao Rathje, George Pinaev, Clarence E Schutt, Uno Lindberg
      European journal of cell biology 87 905-20 2008

      Show Abstract
      18762352 18762352
      Tissue-specific tropomyosin isoform composition.
      Schevzov, Galina, et al.
      J. Histochem. Cytochem., 53: 557-70 (2005) 2005

      Show Abstract
      15872049 15872049
      Sorting of a nonmuscle tropomyosin to a novel cytoskeletal compartment in skeletal muscle results in muscular dystrophy.
      Kee, Anthony J, et al.
      J. Cell Biol., 166: 685-96 (2004) 2004

      Show Abstract
      15337777 15337777
      Tropomyosin localization reveals distinct populations of microfilaments in neurites and growth cones.
      Schevzov, G, et al.
      Mol. Cell. Neurosci., 8: 439-54 (1997) 1997

      Show Abstract
      9143561 9143561

      Data Sheet

      Anti-Tropomyosin - Data Sheet

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