A part of MilliporeSigma

MABC518 | Anti-Trop2 Antibody, clone 2EF, Azide Free

MABC518
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, FC, Inhibition M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC518
      DescriptionAnti-Trop2 Antibody, clone 2EF, Azide Free
      Alternate Names
      • Tumor-associated calcium signal transducer 2
      • Cell surface glycoprotein Trop-2
      • Membrane component chromosome 1 surface marker 1
      • Pancreatic carcinoma marker protein GA733-1
      Background InformationThe protein known as Trop-2, or also known as cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1 or Pancreatic carcinoma marker protein GA733-1 and encoded by the gene TACSTD2/GA733-1/M1S1/TROP2, is a protein that may function as a growth factor receptor and is expressed primarily in the placenta, lung and epithelial tissues but also found in certain cancers. The protein was first identified as a carcinoma antigen by the monoclonal GA733. It is a plasma membrane bound protein that transduces an intracellular calcium signal after binding unknown ligands. Mutations in Trop2 are associated with a rare eye disease leading to blindness. Trop-2 has also recently been shown to be important in regulating fibroblast motility and lung cell proliferation via the regulation of the ERK kinase pathway as well as several critical components of the cell migration apparatus.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing PBS without preservatives.
      Applications
      ApplicationThis Anti-Trop2 Antibody, clone 2EF, Azide Free is validated for use in ICC, flow cytometry & inhibition for the detection of Trop2.
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      • Inhibition
      Application NotesImmunocytochemistry Analysis: A representative lot detected Trop2 in MTE 4-14/T2 and MCF-7 cells (Prof. Saverio Alberti, Oncoxx Biotec.).

      Flow Cytometry Analysis: A representative lot detected Trop2 in transfected cells, and in Skov-3 and HT-29 cancer cells (Prof. Saverio Alberti, Oncoxx Biotec.).

      Inhibition Analysis: A representative lot inhibited tumor growth in DU145 prostate cancer cells, and in HT-29, HCT116 and Colo205 cells. (Note: When used together, Cat. No. MABC518, Anti-Trop2, clone 2EF, Azide Free and Cat. No. MABC519, Anti-Trop2, clone 2G10, Azide Free maximizes inhibition of tumor growth in vivo.) (Prof. Saverio Alberti, Oncoxx Biotec.).
      Biological Information
      ImmunogenRecombinant protein corresponding to the extracellular domain of human Trop2.
      Epitopeextracellular domain
      Clone2EF
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TACSTD2
      • GA733-1
      • M1S1
      • TROP2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight36 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in MET cells transfected with a vector expressing Trop2 or with an empty vector.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Trop2 in MET cells transfected with a vector expressing Trop2 and not in MET cells transfected with empty vector.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalog Number Description  
      ABC425 Anti-Trop-2 Antibody Show Pricing & Availability
      MABC519 Anti-Trop2, clone 2G10, Azide Free Antibody Show Pricing & Availability

      View Gene Related Products for this Target

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies