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MAB4197 | Anti-Topoisomerase II Antibody, clone KiS1

MAB4197
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, WB, ICC, IHC, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB4197
      ReplacesMABE519
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Topoisomerase II Antibody, clone KiS1
      Alternate Names
      • Ki-S1
      Background InformationTopoisomerase II β (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II β is also implicated in drug resistance of tumor cells.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin.
      Applications
      ApplicationAnti-Topoisomerase II Antibody, clone KiS1 is a Mouse Monoclonal Antibody for detection of Topoisomerase II also known as Ki-S1 & has been validated in FC, WB, ICC, IHC, IHC(P).
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1-10μg/ml Immunoprecipitaion

      Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml

      Flow cytometry

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenNuclear protein preparation from the human cell line U937.50
      CloneKiS1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityIn Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
      Gene Symbol
      • TOP2A
      • TOP2
      • TP2A
      • EC 5.99.1.3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11388 # Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
      SIZE: 1531 amino acids; 174385 Da
      SUBUNIT: Homodimer. Interacts with COPS5.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.
      PTM: Phosphorylation has no effect on catalytic activity.
      SIMILARITY: SwissProt: P11388 ## Belongs to the type II topoisomerase family.
      MISCELLANEOUS: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.Avoid repeat freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN TOPOISOMERASE II#945; (Ki-S1) MONOCLONAL ANTIBODY - 2118943 2118943
      MOUSE ANTI-HUMAN TOPOISOMERASE II#945; (Ki-S1) - 2154068 2154068
      MOUSE ANTI-HUMAN TOPOISOMERASE IIa (Ki-S1) MONOCLONAL ANTIBODY - 2036093 2036093
      MOUSE ANTI-HUMAN TOPOISOMERASE IIa (Ki-S1) MONOCLONAL ANTIBODY - 2091732 2091732
      MOUSE ANTI-HUMAN TOPOISOMERASE IIa (Ki-S1) MONOCLONAL ANTIBODY - 2201445 2201445
      MOUSE ANTI-HUMAN TOPOISOMERASE IIa (Ki-S1) MONOCLONAL ANTIBODY - 2295655 2295655
      MOUSE ANTI-HUMAN TOPOISOMERASE IIα (Ki-S1) 2484208
      MOUSE ANTI-HUMAN TOPOISOMERASE IIα (Ki-S1) -2594375 2594375
      MOUSE ANTI-HUMAN TOPOISOMERASE IIα (Ki-S1) -2630173 2630173
      MOUSE ANTI-HUMAN TOPOISOMERASE IIα (Ki-S1) -2691346 2691346
      MOUSE ANTI-HUMAN TOPOISOMERASE IIα (Ki-S1) -2710699 2710699
      MOUSE ANTI-HUMAN TOPOISOMERASE IIα (Ki-S1) -2730933 2730933
      MOUSE ANTI-HUMAN TOPOISOMERASE II (Ki-S1) -2792458 2792458

      References | 11 Available | See All References

      Reference overviewApplicationPub Med ID
      TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution.
      Broderick, R; Nieminuszczy, J; Blackford, AN; Winczura, A; Niedzwiedz, W
      Nature communications 6 6572 2015

      Show Abstract
      25762097 25762097
      Mitotic catenation is monitored and resolved by a PKCε-regulated pathway.
      Brownlow, N; Pike, T; Zicha, D; Collinson, L; Parker, PJ
      Nature communications 5 5685 2014

      Show Abstract
      Western Blotting25483024 25483024
      Deletion of Forkhead Box M1 transcription factor from respiratory epithelial cells inhibits pulmonary tumorigenesis.
      Wang, IC; Meliton, L; Ren, X; Zhang, Y; Balli, D; Snyder, J; Whitsett, JA; Kalinichenko, VV; Kalin, TV
      PloS one 4 e6609 2009

      Show Abstract
      19672312 19672312
      Nuclear reprogramming: the zygotic transcription program is established through an "erase-and-rebuild" strategy.
      Sun, F; Fang, H; Li, R; Gao, T; Zheng, J; Chen, X; Ying, W; Sheng, HZ
      Cell research 17 117-34 2007

      Show Abstract
      17287829 17287829
      DNA double-strand breaks and homology search: inferences from a species with incomplete pairing and synapsis.
      Calvente, A; Viera, A; Page, J; Parra, MT; Gómez, R; Suja, JA; Rufas, JS; Santos, JL
      Journal of cell science 118 2957-63 2005

      Show Abstract
      15976453 15976453
      Ganglioglioma: a detailed histopathological and immunohistochemical analysis of 61 cases.
      Wolf, H K, et al.
      Acta Neuropathol., 88: 166-73 (1994) 1994

      Show Abstract
      7985497 7985497
      The effects of chemotherapy on morphology, cellular proliferation, apoptosis and oncoprotein expression in primary breast carcinoma.
      Rasbridge, S A, et al.
      Br. J. Cancer, 70: 335-41 (1994) 1994

      Show Abstract
      7914426 7914426
      A new proliferation-associated nuclear antigen detectable in paraffin-embedded tissues by the monoclonal antibody Ki-S1.
      Kreipe, H, et al.
      Am. J. Pathol., 142: 3-9 (1993) 1993

      Show Abstract
      8093827 8093827
      Molecular cloning of partial cDNAs for rat DNA topoisomerase II isoforms and their differential expression in brain development.
      Tsutsui, K, et al.
      J. Biol. Chem., 268: 19076-83 (1993) 1993

      Show Abstract
      8395528 8395528
      Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
      Camplejohn, R S, et al.
      Br. J. Cancer, 67: 657-62 (1993) 1993

      Show Abstract
      7682430 7682430

      Data Sheet

      Title
      Anti-Topoisomerase II, clone KiS1 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies