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MABF73 | Anti-Tim3 Antibody, clone 5D12

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC M Purified Monoclonal Antibody
      Catalogue NumberMABF73
      DescriptionAnti-Tim3 Antibody, clone 5D12
      Alternate Names
      • Hepatitis A virus cellular receptor 2 homolog
      • T-cell immunoglobulin and mucin domain-containing protein 3
      • T-cell membrane protein 3
      • TIMD-3
      • TIM-3
      Background InformationAs a member of the T cell immunoglobulin and mucin domain (TIM) family, Tim-3 is reported to promote or terminate Th1 immunity and is thought to influence a range of inflammatory conditions. It is constitutively expressed in a subset of murine IFNgamma-secreting Th1 cells, but not Th2 cells, in both human and mice. Additionally, Tim-3 is expressed dendritic cells, and by a subset of CD4+ cells where its levels of expression increase upon anti-CD3/28 stimulation. This molecule is also be highly expressed in in vitro polarized Th1 cells, and at lower levels in Th17 cells. Tim-3 is known to synergize with Toll-like receptors (TLRs) and is thought to regulate Th1 and Th17 cytokine secretion.
      Product Information
      • RAW264.7 cells
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Tim3 Antibody, clone 5D12 is an antibody against Tim3 for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenRecombinant protein corresponding to full length mouse Tim3.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.
      Gene Symbol
      • Havcr2
      • Tim3
      • Timd3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      Molecular Weight31 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in RAW264.7 cells.

      Flow Cytometry Analysis: A 1:50 dilution of this antibody detected Tim3 in RAW264.7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tim3, clone 5D12 - 2432518 2432518
      Anti-Tim3, clone 5D12 - 2279274 2279274
      Anti-Tim3, clone 5D12 - 2510397 2510397
      Anti-Tim3, clone 5D12 - Q1966976 Q1966976
      Anti-Tim3, clone 5D12 -2605471 2605471
      Anti-Tim3, clone 5D12 -2608855 2608855
      Anti-Tim3, clone 5D12 -2631293 2631293
      Anti-Tim3, clone 5D12 -2702178 2702178
      Anti-Tim3, clone 5D12 -2732599 2732599
      Anti-Tim3, clone 5D12 -2769076 2769076


      Reference overviewPub Med ID
      CEACAM1 regulates TIM-3-mediated tolerance and exhaustion.
      Huang, YH; Zhu, C; Kondo, Y; Anderson, AC; Gandhi, A; Russell, A; Dougan, SK; Petersen, BS; Melum, E; Pertel, T; Clayton, KL; Raab, M; Chen, Q; Beauchemin, N; Yazaki, PJ; Pyzik, M; Ostrowski, MA; Glickman, JN; Rudd, CE; Ploegh, HL; Franke, A; Petsko, GA; Kuchroo, VK; Blumberg, RS
      Nature 517 386-90 2015

      Show Abstract
      25363763 25363763

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      Life Science Research > Antibodies and Assays > Primary Antibodies