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610205 | Anti-Tie2 Mouse mAb (Ab33)

610205
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, Porcine, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      610205-50UG
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      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 50 μg
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          Description
          OverviewRecognizes the ~140-45 kDa Tie2 protein.
          Catalogue Number610205
          Brand Family Calbiochem®
          SynonymsAnti-Angiopoietin Receptor, Anti-Tek
          References
          ReferencesLin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
          Wong, A.L., et al. 1997. Circ. Res. 81, 567.
          Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Not Paraffin Sections
          Application NotesFrozen Sections (see comments)
          Immunoblotting (0.2-1 µg/ml, chemiluminescence)
          Immunoprecipitation (see comments)
          Paraffin Sections (not recommended)
          Application CommentsFor frozen sections, tissues were treated with Triton®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag® sequence
          ImmunogenHuman
          CloneAb33
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Porcine
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          610205

          References

          Reference overview
          Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
          Wong, A.L., et al. 1997. Circ. Res. 81, 567.
          Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-August-2007 RFH
          SynonymsAnti-Angiopoietin Receptor, Anti-Tek
          ApplicationFrozen Sections (see comments)
          Immunoblotting (0.2-1 µg/ml, chemiluminescence)
          Immunoprecipitation (see comments)
          Paraffin Sections (not recommended)
          DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
          BackgroundAngiopoietin-1 and its naturally occurring inhibitor, angiopoietin-2, exert their effects by inducing autophosphorylation of their signaling receptor Tie2. Phosphorylation of the receptor occurs during vasculogenesis and is required for its activity in regulating endothelial cell proliferation, differentiation and guiding the proper patterning of endothelial cells during blood vessel formation. Tie-2 is a transmembrane receptor-tyrosine kinase that is expressed by endothelial cells and primitive hematopoietic tissues. Tie2/Tek has been shown to be critical for endothelial cell survival, which is evidenced in part by the constitutive phosphorylation that is observed in quiescent endothelium. More recently a multifunction docking site has been identified on the receptor (Tyr1100) that serves as a binding site for other partners including Grb2, Grb7, and p85 and acting via an SH2 domain interaction.
          HostMouse
          Immunogen speciesHuman
          Immunogena recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag® sequence
          CloneAb33
          IsotypeIgG₁
          Specieshuman, mouse, porcine, rat
          FormLiquid
          FormulationIn 150 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsFor frozen sections, tissues were treated with Triton®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesLin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
          Wong, A.L., et al. 1997. Circ. Res. 81, 567.
          Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.