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MAB1148 | Anti-Tie-2 Antibody, extracellular, clone 1E11DH

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M ELISA, FC, IP, WB R Purified Monoclonal Antibody
      Catalogue NumberMAB1148
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tie-2 Antibody, extracellular, clone 1E11DH
      OverviewPurity: Greater than 95% by SDS-PAGE. 0.22 micron sterile filtered.
      Alternate Names
      • Angiopoietin-1 Receptor
      • p140 TEK
      Background InformationTie-2 is an endothelial-specific receptor tyrosine kinase and a receptor for angiopoietin 1. It is a type I membrane protein that is expressed predominantly in the endothelium of actively growing blood vessels and may represent the earliest mammalian endothelial cell lineage marker. Tie-2 is likely involved in the regulation of endothelial cell proliferation and differentiation and may direct the special orientation of endothelial cells during the formation of blood vessels.
      Product Information
      • POSITIVE CONTROL: placenta and lung tissue.
      PresentationImmunoglobulin purified by protein G affinity chromatography. Liquid in PBS, pH 7.4, containing 0.05% Bronidox as a preservative.
      ApplicationDetect Tie-2 using this Anti-Tie-2 Antibody, extracellular, clone 1E11DH validated for use in ELISA, FC, IP & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 5 μg/mL. Recognizes a band migrating at approximately 140 kDa corresponding to mouse Tie-2.

      FACS: 10 μg/mL

      Immunoprecipitation: 10 μg/mL of lysate


      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenThe extracellular domain of mouse Tie-2 receptor.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes mouse Tie-2 Receptor.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
      Gene Symbol
      • TEK
      • CD202B
      • TIE2
      • VMCM1
      • VMCM
      • hTIE2
      • CD202b
      • TIE-2
      • EC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q02763 # This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
      SIZE: 1124 amino acids; 125811 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
      DISEASE:SwissProt: Q02763 # Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
      SIMILARITY: SwissProt: Q02763 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. & Contains 3 EGF-like domains. & Contains 3 fibronectin type-III domains. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti Tie-2 (1E11DH) - Rat Monoclonal (100ug) - ME9EN5633 ME9EN5633
      Anti Tie-2 (1E11DH) - Rat Monoclonal (100ug) - ME9JN2333 ME9JN2333


      Reference overviewPub Med ID
      HOXA13 Is essential for placental vascular patterning and labyrinth endothelial specification.
      Shaut, CA; Keene, DR; Sorensen, LK; Li, DY; Stadler, HS
      PLoS genetics 4 e1000073 2008

      Show Abstract Full Text Article
      18483557 18483557
      Tie2 receptor expression and phosphorylation in cultured cells and mouse tissues.
      Koblizek, T I, et al.
      Eur. J. Biochem., 244: 774-9 (1997) 1997

      Show Abstract
      9108247 9108247
      tie2, a putative protein tyrosine kinase from a new class of cell surface receptor.
      Runting, A S, et al.
      Growth Factors, 9: 99-105 (1993) 1993

      Show Abstract
      8217221 8217221

      Data Sheet

      Anti-Tie-2, extracellular, clone 1E11DH - Data Sheet