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MABE370 | Anti-Thymidylate Synthase-5FU complex Antibody, clone FTS

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB R Purified Monoclonal Antibody
      Catalogue NumberMABE370
      DescriptionAnti-Thymidylate Synthase-5FU complex Antibody, clone FTS
      Alternate Names
      • Thymidylate synthase
      • TS
      • Tsase
      Background InformationThymidylate Synthase (TS), a cytosolic enzyme, is a dimer of two identical monomers of about 36kDa. The enzyme provides the sole intracellular de novo source of thymidylate and plays a crucial role in DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dump) and its conversion to deoxythymidine monophosphate (dTMP). Therefore, TS is primarily active in proliferating and metabolic active cells. TS is a central target of the widely used antineoplastic agent 5-Fluorouracil (5-FU) and thus also of the Xeloda, which is enzymatically activated to 5-FU. TS is inactivated by a covalent complex formation with 5-FdUMP and methylenetetrahydrofolate. Literature indicates that expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas with low TS expression predicting better response to 5-FU and survival.
      Product Information
      • 5FU treated RKO cell lysate
      PresentationPurified rat monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect Thymidylate Synthase using this rat monoclonal antibody, Anti-Thymidylate Synthase-5FU complex Antibody & clone FTS validated for use in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenCorresponding to human Thymidylate Synthase-5FU complex.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody demonstrates specificity against Thymidylate Synthase-5FU complex , and does not cross react with Thymidylate Synthase (Patel, K., et al. (2012). Oncotarget. 3(7):678-685).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TYMS
      • TS
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~36 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RKO cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Thymidylate Synthase-5FU complex in 10 µg of RKO cell lysates treated with 5 FU, and demonstrated a loss of signal in untreated RKO cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies